AB10521

Anti-Neuropilin-1 Antibody

serum, from rabbit

• Synonym(s): CD304 antigen, Vascular endothelial cell growth factor 165 receptor, neuropilin 1, transmembrane receptor, Npn 1
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: serum
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: rat, mouse
• species reactivity (predicted by homology): human (94%)
• technique(s): immunofluorescence: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• isotype: IgG
• NCBI accession no.: NP_659566.1
• UniProt accession no.: Q9QWJ9
• shipped in: wet ice
• Gene Information: human ... NRP1(8829)

Description

General description

Neuropilin-1 is a type 1 membrane protein with three distinct functions. First, it can mediate cell adhesion via a heterophilic molecular interaction. Second, in neuronal cells, neuropilin-1 binds the class 3 semaphorins, which are neuronal chemorepellents, and plays a role in the directional guidance of axons. It is expected to form complexes with the plexinA subfamily members and mediate the semaphorin-elicited inhibitory signals into neurons. Third, in endothelial cells, neuropilin-1 binds a potent endothelial cell mitogen, vascular endothelial growth factor (VEGF)165, and regulates vessel formation.

Specificity

This antibody recognizes Neuropilin-1.

Immunogen

Epitope: Unknown

This antibody is generated from rabbits immunized with a His-tagged partial recombinant rat Neuropilin-1.

Application

Research Category
Neuroscience

Research Sub Category
Growth Cones & Axon Guidance

This Anti-Neuropilin-1 Antibody is validated for use in WB, IH, IP, IF for the detection of Neuropilin-1.

Quality

Evaluated by Western Blot in PC12 cell lysate.
Western Blot Analysis: : 1:1,000 dilution of this antibody detected Neuropilin 1 on 10 µg of PC12 cell lysate.

Target description

130 kDa

Physical form

Rabbit serum in buffer containing 0.05% sodium azide.

Unpurified

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
PC12 cell lysate

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1