AB1284

Sigma-Aldrich

Anti-Heme Oxygenase 1 Antibody

Chemicon®, from rabbit

Synonym(s):
HO-1, HSP32
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse, bovine, rat

should not react with

canine

manufacturer/tradename

Chemicon®

application(s)

immunohistochemistry: suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

dry ice

Specificity

Recognizes Heme oxygenase-1.

Immunogen

A synthetic peptide with the amino acid sequence H-D-L-S-E-A-L-K-E-A-T-K-E-V-H-C-NH2 corresponding to amino acid residues [12-25] + Cys-NH2 of the human HO-1 protein (Yoshida et al., 1988).

Application

This Anti-Heme Oxygenase 1 Antibody is validated for use in IH, WB for the detection of Heme Oxygenase 1.
Western Blot: 1:1,000 (using ECL). A single band of ~32 kD is observed. Reactivity can be completely abolished by pre-adsorption of the antibody with the complementary peptide (Catalog Number AG253). Also available are microsomal tissue preparations for control purposes (Rat spleen microsomal preparation, Catalog Number AG257).

Immunohistochemistry: 1:50-1:1,000 on human airway biopsy, human spleen, human kidney and porcine arteries.

Optimal working dilutions must be determined by the end user.
Research Category
Metabolism
Research Sub Category
Toxicology & Drug Resistance

Target description

32 kDa

Physical form

Affinity Purified immunoglobulin. Liquid in PBS containing 0.01M sodium azide.
ImmunoAffinity Purified

Storage and Stability

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Lung

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Resveratrol increases vascular oxidative stress resistance.
Ungvari, Z; Orosz, Z; Rivera, A; Labinskyy, N; Xiangmin, Z; Olson, S; Podlutsky, A; Csiszar, A
American Journal of Physiology. Heart and Circulatory Physiology null
Purification and properties of heme oxygenase from pig spleen microsomes
Yoshida T, and Kikuchi G.
The Journal of Biological Chemistry, 253, 4224-4229 (1978)
Solubilization and partial purification of heme oxygenase from rat liver.
Maines, M D, et al.
The Journal of Biological Chemistry, 252, 5900-5903 (1977)
T Yoshida et al.
European journal of biochemistry, 171(3), 457-461 (1988-02-01)
Hemin treatment increased both activity and mRNA level of heme oxygenase in human macrophages. Using poly(A)-rich RNA prepared from human macrophages treated with hemin, we have constructed a cDNA library in the Okayama-Berg vector. The human heme oxygenase cDNA was...
Up-regulation of heme oxygenase-1 by isoflurane preconditioning during tolerance against neuronal injury induced by oxygen glucose deprivation.
Qifang Li, Yesen Zhu, Hong Jiang, Hui Xu, Heping Liu
Acta biochimica et biophysica Sinica null

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