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Key Documents

AB1344

Sigma-Aldrich

Anti-Glucose Transporter GLUT-3 Antibody, CT

serum, Chemicon®

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

rat, mouse

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

Specificity

Recognizes Glut-3 in mouse and rat tissues. C terminal peptide used shows no homology to the human sequence.

Immunogen

Synthetic peptide corresponding to the C-terminus of mouse Glut-3 coupled to KLH. Product sold sold as AG617

Application

Anti-Glucose Transporter GLUT-3 Antibody, C-terminus is an antibody against Glucose Transporter GLUT-3 for use in ELISA & WB.
Western blotting: 1:1,000-1:5,000, using chemiluminescent detection. Detects a 45-48kDa band in membrane preps from rat brain. Slight differences in band sizes in different tissues; reported range is 45-55kDa.

Other detection methods may require a higher concentration of antibody.

ELISA: 1:10,000 - 1:50,000, using 50-100 ng of antigen/well.

Optimal working dilutions must be determined by end user.

Physical form

Liquid rabbit antiserum containing 0.05% sodium azide.

Storage and Stability

Store at -20°C for up to 12 months after date of receipt in undiluted aliquots. Avoid repeated freeze-thaw cycles.

Analysis Note

Control
The control peptide is available for absorbtion studies. Please see catalog number AG617.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 1


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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C M Cheng et al.
Proceedings of the National Academy of Sciences of the United States of America, 97(18), 10236-10241 (2000-08-24)
The brain has enormous anabolic needs during early postnatal development. This study presents multiple lines of evidence showing that endogenous brain insulin-like growth factor 1 (Igf1) serves an essential, insulin-like role in promoting neuronal glucose utilization and growth during this
Sze Ting Cecilia Kwan et al.
The Journal of nutrition, 147(11), 2083-2092 (2017-09-22)
Background: Fetal growth is dependent on placental nutrient supply, which is influenced by placental perfusion and transporter abundance. Previous research indicates that adequate choline nutrition during pregnancy improves placental vascular development, supporting the hypothesis that choline may affect placental nutrient
Cinzia Dello Russo et al.
The Journal of biological chemistry, 278(8), 5828-5836 (2002-12-18)
Activation of peroxisome proliferator-activated receptors (PPARs) can regulate brain physiology and provide protection in models of neurological disease; however, neither their exact targets nor mechanisms of action in brain are known. In many cells, PPAR gamma agonists increase glucose uptake
Raimund I Herzog et al.
The Journal of clinical investigation, 123(5), 1988-1998 (2013-04-02)
Hypoglycemia occurs frequently during intensive insulin therapy in patients with both type 1 and type 2 diabetes and remains the single most important obstacle in achieving tight glycemic control. Using a rodent model of hypoglycemia, we demonstrated that exposure to
Jean-Sébastien Joyal et al.
Nature medicine, 22(4), 439-445 (2016-03-15)
Tissues with high metabolic rates often use lipids, as well as glucose, for energy, conferring a survival advantage during feast and famine. Current dogma suggests that high-energy-consuming photoreceptors depend on glucose. Here we show that the retina also uses fatty

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