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AB144P

Sigma-Aldrich

Anti-Choline Acetyltransferase Antibody

Chemicon®, from goat

Synonym(s):
Choline acetylase, acetyl CoA:choline O-acetyltransferase, choline acetyltransferase, ChAT
eCl@ss:
32160702
NACRES:
NA.41

Quality Level

biological source

goat

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

guinea pig, mouse, opossum, zebrafish (THE JOURNAL OF COMPARATIVE NEUROLOGY 474:75–107 (2004).), avian, rat, chicken, monkey, human

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

dry ice

Gene Information

chicken ... Chat(395314)
human ... CHAT(1103)
mouse ... Chat(12647)
rat ... Chat(290567)
rhesus monkey ... Chat(709977)

General description

Choline acetyltransferase (ChAT) has a molecular weight of about 68 kDa. It is synthesized in the parakaryon and is transported to the axon terminals. ChAT belongs to the choline/carnitine acyltransferase family. It seems that only cholinergic neurons have significant amounts of ChAT making anti-choline acetyltransferase a useful specific marker. Choline acetyltransferase is found in cholinergic neurons in brain and spinal cord.

Specificity

Expected to cross-react with Avian, chicken, guinea pig, monkey, rat, and opposum.

Immunogen

Human placental enzyme.

Application

Anti-Choline Acetyltransferase Antibody has been used in:
  • immunohistochemical staining
  • western blotting
  • immunocytochemistry

Immunohistochemistry:
A previous lot of this antibody was used at 1:100.

Optimal working dilutions and protocols must be determined by end user.

Biochem/physiol Actions

Choline acetyltransferase (CHAT) is involved in the synthesis of acetylcholine in neurons. It catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. CHAT catalyzes the transfer of an acetyl group between acetyl-coenzyme A and choline. Mutations in the gene encoding this protein have been associated with congenital neuromuscular disorders.

Quality

Routinely evaluated by Western Blot on mouse brain lysates.

Western Blot Analysis:
1:1000 dilution of this lot detected CHAT on 10 μg of mouse brain lysates.

Target description

70/74 kDa

Physical form

Affinity purified
Purified goat polyclonal in buffer containing 5 mg/mL BSA and 0.2% sodium azide.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 1 year from date of receipt. Avoid repeated freeze/thaw cycles.
For short term storage (3-4 weeks) maintain at 2-8°C.

Analysis Note

Control
Human placenta lysates, rat forebrain/rostral hypothalamus.

Other Notes

Research Category:
Neuroscience

Research Sub Category:
Neurotransmitters & Receptors

Neuronal & Glial Markers

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Quality

Enter Lot Number to search for Certificate of Quality (COQ).

Choline acetyltransferase structure reveals distribution of mutations that cause motor disorders
Cai Y, et al.
The Embo Journal, 2047-2047 (2004)
Blood pressure regulation by CD4+lymphocytes expressing choline acetyltransferase
Olofsson PS, et al.
Nature Biotechnology, 34(10), 1066-1071 (2016)
Plasticity of motor network and function in the absence of corticospinal projection
Han Q, et al.
Experimental Neurology, 194-208 (2015)
Cholinergic modulation of synaptic transmission and postsynaptic excitability in the rat gracilis dorsal column nucleus.
Fernandez de Sevilla, D; Rodrigo-Angulo, M; Nu?ez, A; Bu?o, W
The Journal of Neuroscience null
The human choline acetyltransferase gene encodes two proteins
Grosman DD, et al.
Journal of Neurochemistry (1995)

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