AB1498P

Sigma-Aldrich

Anti-Stem Cell Factor Antibody

Chemicon®, from rabbit

Synonym(s):
SCF, c-kit Ligand, Mast Cell Growth Factor, MGF
eCl@ss:
32160702
NACRES:
NA.41
Pricing and availability is not currently available.

biological source

rabbit

Quality Level

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse

mfr. no.

Chemicon®

application(s)

ELISA: suitable
neutralization: suitable
western blot: suitable

input

sample type hematopoietic stem cell(s)

NCBI accession no.

UniProt accession no.

shipped in

wet ice

Gene Information

human ... KITLG(4254)

General description

Stem Cell Factor (SCF), also known as c-Kit ligand (KL), steel factor (SLF) and mast cell growth factor (MGF), is a 30 kDa glycoprotein with broad activities on various tissues, including hematopoietic cells, pigment cells, and primordial germ cells. SCF is secreted by endothelial cells, fibroblasts, and bone marrow stromal cells as a membrane-bound form which may be cleaved to release the soluble form. Both forms are active in promoting colony formation from murine bone marrow cells, but membrane-bound SCF is more effective in promoting hematopoieses in vivo, suggesting a role in cellular interactions between hematopoietic and stromal cells. The soluble form is thought to exist in solution as a noncovalently linked dimer. SCF is structurally related to M-CSF (CSF-1) and Flt-3/Flk-2 Ligand (FL) with all three sharing a similar size, existence of transmembrane and soluble forms, four conserved cysteines, and alternative splicing exon locations, but they share little sequence homology. SCF alone is a modest colony stimulating factor. However, in the presence of other cytokines such as EPO, TPO, GM-CSF, G-CSF, M-CSF, IL-3, and IL-7, SCF is a potent costimulant that works synergistically to increase the size of myeloid, erythroid or lymphoid lineage colonies without influencing the lineage differentiation of the progenitors.

Specificity

Reacts with mouse SCF; SCF is highly conserved cross reactivity with rat, human and other species SCF is expected but untested.

Immunogen

Highly pure (>98%) recombinant mouse SCF

Application

Research Sub Category
Hematopoietic Stem Cells
Detect Stem Cell Factor using this Anti-Stem Cell Factor Antibody validated for use in ELISA, WB, NEUT.
Research Category
Stem Cell Research
ELISA: To detect mSCF by direct ELISA (using 100 μL/well antibody solution) a concentration of at lease 0.5 μg/mL of this antibody is required. Should allow detection of 0.2 - 0.4 ng/well of recombinant mSCF.

Neutralization: For ½ maximal inhibition (ND50) of the bioogical activity of mSCF (20 ng/mL), a concentration of 0.64 - 0.97 μg/mL is required.

Western blot: 0.1 - 0.2 μg/mL, this dilution should offer a detection limit of 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Optimal working dilutions must be determined by the end user.

Target description

30 kDa

Physical form

Affinity purified immunoglobulin. Lyophilized from 0.5X PBS pH 7.2. Reconstitute with sterile distilled water.

Storage and Stability

The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20ºC. When reconstituted in sterile water to a concentration of = 0.2 mg/ml the antibody is stable for at least six weeks at 2-4ºC.

Analysis Note

Control
Human mast cells

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Blocking SCF/c-Kit signal did not inhibit the proliferation of cultured liver progenitor cells.
Caixia Jin,Wenlin Li,Fang Xu,Ji Zhu,Zhiying He,Yiping Hu
IUBMB Life null
Lixin Sun et al.
The Journal of clinical investigation, 113(9), 1364-1374 (2004-05-05)
Neural stem/progenitor cell (NSPC) migration toward sites of damaged central nervous system (CNS) tissue may represent an adaptive response for the purpose of limiting and/or repairing damage. Little is known of the mechanisms responsible for this migratory response. We constructed...
Acral lesions of vitiligo: why are they resistant to photochemotherapy?
S M Esmat,A M El-Tawdy,G A Hafez,O A Zeid,D M Abdel Halim,M A Saleh,T M Leheta,M Elmofty
Journal of the European Academy of Dermatology and Venereology : JEADV null

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