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AB1504

Sigma-Aldrich

Anti-Glutamate receptor 1 Antibody

from rabbit, purified by affinity chromatography

Synonym(s):
AMPA 1, AMPA-selective glutamate receptor 1, Glutamate receptor ionotropic, AMPA 1, glutamate receptor, ionotropic, AMPA 1
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse, horse, rat, human

species reactivity (predicted by homology)

equine (based on 100% sequence homology), platypus (based on 100% sequence homology)

technique(s)

immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

Gene Information

human ... GRIA1(2890)

General description

Glutamate receptors (GluRs) can be categorized as ionotropic or metabotropic and subcatergorized by their agonist preferences (NMDA, AMPA or Kainic acid). There are four types of AMPA selective GluR subunits (GluR1, GluR2, GluR3 and GluR4). Tetrameric or pentameric combinations of different subunits contributes to the functional diversity of AMPA receptors. In general, AMPA receptors mediate fast synaptic current at most excitatory synapses, with stoichiometry characterized by subtype composition. GluR1 is believed to mediate calcium influx, and has a vital function in synaptic plasticity related to learning. Reduction in GluR1 has been caused by FMR1 gene mutation and contributes to fragile X syndrome. Changes in expression of GluR1 receptors on dopamine neurons have been correlated with sensitization of drugs of abuse.

Specificity

This antibody recognizes Glutamate receptor 1 at the cytoplasmic domain.

Immunogen

Epitope: Cytoplasmic domain
KLH-conjugated linear peptide corresponding to human Glutamate receptor 1 at the cytoplasmic domain.

Application

Research Category
Neuroscience
Immunohistochemistry Analysis: 1:300 dilution of this antibody has been shown to detect Glutamate receptor 1 (GluR1) in rat brain tissue.
This Anti-Glutamate receptor 1 Antibody is validated for use in WB, IH(P), IP for the detection of Glutamate receptor 1.
Research Sub Category
Neurotransmitters & Receptors

Quality

Evaluated by Western Blot in mouse brain lysate.

Western Blot Analysis: 1:1000 dilution of this antibody detected Glutamate receptor 1 (GluR1) on 10 µg of mouse brain lysate.

Target description

Observed at ~106 kDa

Physical form

Affinity purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Mouse brain lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Quality

Enter Lot Number to search for Certificate of Quality (COQ).

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