AB1513P

Sigma-Aldrich

Anti-Brain Derived Neurotrophic Factor Antibody

Chemicon®, from sheep

Synonym(s):
BDNF
eCl@ss:
32160702
NACRES:
NA.41

Quality Level

biological source

sheep

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human, rat, chicken

manufacturer/tradename

Chemicon®

application(s)

ELISA: suitable
immunohistochemistry: suitable
western blot: suitable

UniProt accession no.

shipped in

dry ice

Gene Information

human ... BDNF(627)

Specificity

Recognizes Brain Derived Neurotrophic Factor (BDNF). Neutralizes BDNF, but not other neurotrophins. By one site ELISA, less than 1% cross-reactivity against mouse NGF, recombinant human NT3 or neurotrophin 4. The antibody was tested on human and rat derived samples, but based on sequence similarity it is expected to react with mouse and other mammalian species. The peptide sequences of human, rat and mouse BDNF are identical.

Immunogen

Recombinant Human Brain Derived Neurotrophic Factor (BDNF).

Application

Research Sub Category
Neurochemistry & Neurotrophins

Neuroinflammation & Pain
Anti-Brain Derived Neurotrophic Factor Antibody detects level of Brain Derived Neurotrophic Factor & has been published & validated for use in ELISA, IH, FUNC & WB.
Research Category
Neuroscience
Immunohistochemistry: 1-5 μg/mL (see suggested protocol).

Immunoblotting: 1-5 μg/mL we recommend preparing the BDNF samples for western blot by the method of Semba-Katoh, R et.al (J. Neurochem. 69(1):34-42, 1997) because BDNF is not easily extracted in standard buffers. Dissected tissues should be homogenized in 10 volumes of 100mM phosphate buffer containing 1mM EDTA, 2M guanidine hydrochloride (pH7.2), and three protease inhibitors, 10mM N-ethylmaleimide, 0.36mM pepstatin, and 1mM PMSF. Homogenates are then sonicated and centrifuged at 46K x g for 30 minutes at 4°C. BDNF migrates as ~12-18kDa monomer, with a 28-30kDa homodimer in most samples.

ELISA: 1-5 μg/mL

Inhibition of biological activity in vitro: Complete inhibition of BDNF activity on embryonic chick sensory neurons occurs at 1-5 μg/mL. Biological inhibition protocols can be found in Finn et al. (J. Neurocytol. 15:169-176, 1986).
BIOLOGICAL ACTIVITY: Neutralizes BDNF, but not other neurotrophins.

Use neat for in vivo studies.

Optimal working dilutions must be determined by end user.

Target description

~12-18kDa monomer, with a 28-30kDa homodimer in most samples.

Physical form

Protein G Purified
Protein G Purified immunoglobulin. Lyophilized from PBS, no preservatives. Reconstitute with 500 μL of sterile distilled water.
Format: Purified

Storage and Stability

Maintain lyophilized material at -20 to -70°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Glycerol (1:1) can be added for additional stability. Avoid repeated freeze/thaw cycles.

Analysis Note

Control
Brain tissue

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Mariana Alonso et al.
Learning & memory (Cold Spring Harbor, N.Y.), 12(5), 504-510 (2005-10-06)
Information storage in the brain is a temporally graded process involving different memory phases as well as different structures in the mammalian brain. Cortical plasticity seems to be essential to store stable long-term memories, although little information is available at...
Brain-derived neurotrophic factor augments peristalsis by augmenting 5-HT and calcitonin gene-related peptide release.
Grider John R JR, Piland Barbara E BE, Gulick Melisa A MA, Qiao Li Ya LY
Gastroenterology null
Feedback mechanism in depolarization-induced sustained activation of extracellular signal-regulated kinase in the hippocampus.
Maharana, C; Sharma, KP; Sharma, SK
Scientific Reports null
Jennifer C Kasemeier-Kulesa et al.
Nature communications, 6, 8281-8281 (2015-09-26)
The sympathetic nervous system is essential for maintaining mammalian homeostasis. How this intricately connected network, composed of preganglionic neurons that reside in the spinal cord and post-ganglionic neurons that comprise a chain of vertebral sympathetic ganglia, arises developmentally is incompletely...
M B Young et al.
Translational psychiatry, 5, e634-e634 (2015-09-16)
Acutely administered 3,4-methylenedioxymethamphetamine (MDMA, 'ecstasy') has been proposed to have long-term positive effects on post-traumatic stress disorder (PTSD) symptoms when combined with psychotherapy. No preclinical data support a mechanistic basis for these claims. Given the persistent nature of psychotherapeutic gains...

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