AB152

Sigma-Aldrich

Anti-Tyrosine Hydroxylase Antibody

Chemicon®, from rabbit

Synonym(s):
Tyrosine 3-hydroxylase, Tyrosine 3-monooxygenase, tyrosine hydroxylase, TH
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse, mollusk, feline, squid, human, ferret, rat, Aplysia (marine mollusc), Drosophila

manufacturer/tradename

Chemicon®

application(s)

ELISA: suitable
immunofluorescence: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

dry ice

General description

Tyrosine hydroxylase (also know as Tyrosine 3-hydroxylase and Tyrosine 3-monooxygenase) plays an important role in the physiology of adrenergic neurons. It is the first and rate-limiting enzyme involved in the biosynthesis of the catecholamines Dopamine and Norepinephrine from tyrosine. TH is, therefore, a useful marker for dopaminergic and noradrenergic neurons. The enzymatic activity of TH requires ferrous ions as cofactors and is believed to be regulated by phosphorylation. At least four isoforms of human TH have been identified which result from alternative splicing.

Specificity

It is expected that the antibody will react with most mammalian and many non-mammalian species. It has been reported that this antibody does not work on paraffin embedded human tissue.
Tyrosine hydroxylase. By western blot, AB152 selective labels a single band at approximately 62kDa (reduced) corresponding to Tyrosine Hydroxylase. It is expected that the antibody will react with most mammalian and many non-mammalian species. It has been reported that this antibody does not work on paraffin embedded human tissue.

Immunogen

Denatured tyrosine hydroxylase from rat pheochromocytoma (denatured by sodium dodecyl sulfate).

Application

Anti-Tyrosine Hydroxylase Antibody detects level of TH and has been published and validated for use in ELISA, IF, IH, IH(P), IP and WB.
Research Sub Category
Neurotransmitters & Receptors

Neuronal & Glial Markers
Research Category
Neuroscience
Immunohistochemistry:
A previous lot of this antibody was uesd at 1:100-1:1,000 dilution, 4°C, for 1-3 days. Stains both fresh frozen and paraffin embedded tissue samples by indirect immunofluorescence and immunoperoxidase.

Immunofluorescence:
A previous lot of this antibody was used at 1:100-1:1,000 dilution.

Immunoprecipitation:
A previous lot of this antibody was used on Immunoprecipitation.

ELISA:
A previous lot of this antibody was used in ELISA.

Optimal dilutions must be determined by the end user.

Quality

Routinely evaluated by Western Blot on PC12 lysates.

Western Blot Analysis:
1:1000 dilution of this lot detected tyrosine hydroxylase on 10 μg of PC12 lysates.

Target description

62 kDa

Physical form

Purified rabbit polyclonal in buffer containing liquid in 10 mM HEPES (pH 7.5), 150 mM NaCl (pH 7.5), 100 ug/mL BSA and 50% glycerol.
Affinity purified

Storage and Stability

Maintain frozen at -20ºC to -70ºC in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. Do not store in a self defrosting freezer.

Analysis Note

Control
POSITIVE CONTROL: Brain (corpus striatum, sympathetic nerve terminals) and adrenal glands.
NEGATATIVE CONTROL: Liver.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

storage_class_code

10 - Combustible liquids

WGK Germany

WGK 2

Certificate of Analysis

Certificate of Origin

Low density of sympathetic nerve fibers relative to substance P-positive nerve fibers in lesional skin of chronic pruritus and prurigo nodularis.
Haas S, Capellino S, Phan NQ, Bohm M, Luger TA, Straub RH, Stander S
Journal of Dermatological Science null
Axon guidance of mouse olfactory sensory neurons by odorant receptors and the beta2 adrenergic receptor.
Feinstein, P; Bozza, T; Rodriguez, I; Vassalli, A; Mombaerts, P
Cell null
Autocrine VEGF-VEGF-R loop on podocytes during glomerulonephritis in humans.
Hohenstein B, Colin M, Foellmer C, Amann KU, Brekken RA, Daniel C, Hugo CP
Nephrology, Dialysis, and Transplantation null
Characterisation of behavioural and neurodegenerative changes induced by intranigral 6-hydroxydopamine lesions in a mouse model of Parkinson's disease.
Grealish S, Mattsson B, Draxler P, Bjorklund A
The European Journal of Neuroscience null
Induction of FosB/DeltaFosB in the brain stress system-related structures during morphine dependence and withdrawal.
Nu?ez C, Martin F, Foldes A, Luisa Laorden M, Kovacs KJ, Victoria Milanes M
Journal of Neurochemistry null
Articles
Human iPSC neural differentiation media and protocols used to generate neural stem cells, neurons and glial cell types.
Read More

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service