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  • AB15282
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AB15282

Sigma-Aldrich

Anti-Cone Arrestin Antibody

Chemicon®, from rabbit

Synonym(s):
Anti-Arrestin-C
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

rat, mouse

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry: suitable (paraffin)
western blot: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

wet ice

Gene Information

mouse ... Arr3(170735)
rat ... Arr3(171107)

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biological source

mouse

biological source

rabbit

biological source

rabbit

antibody form

affinity isolated antibody

antibody form

ascites fluid

antibody form

affinity isolated antibody

antibody form

affinity purified immunoglobulin

clone

polyclonal

clone

monoclonal

clone

polyclonal

clone

polyclonal

species reactivity

rat, mouse

species reactivity

rat, human, pig, bovine, goat, sheep

species reactivity

mouse

species reactivity

human

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

manufacturer/tradename

-

manufacturer/tradename

Upstate®

technique(s)

immunohistochemistry: suitable (paraffin), western blot: suitable

technique(s)

immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

immunofluorescence: suitable, immunohistochemistry: suitable, western blot: suitable

technique(s)

western blot: suitable

General description

Arrestin-C (UniProt: Q9EQP6; also known as Cone arrestin, cArr, Retinal cone arrestin-3) is encoded by the Arr3 gene (Gene ID: 170735) in murine species. Arrestins are a superfamily of multi-functional proteins that that regulate signaling and trafficking of the majority of G protein-coupled receptors (GPCRs), as well as sub-cellular localization and activity of many other signaling proteins. Arrestin-C is predominantly found in Inner and outer segments, and the inner plexiform regions of the retina. It is expressed in cone photoreceptors and pinealocytes and may contribute to the shut-off mechanisms associated with high acuity color vision. Arrestin-C is an elongated two-domain molecule with overall fold and key inter-domain interactions that hold the free protein in the basal conformation similar to the other subtypes. Several structural elements are reported to contribute to arrestin binding. The C-terminal acidic region serves a regulatory role in controlling arrestin binding selectivity toward the phosphorylated and activated form of a receptor. The basic N-terminal domain directly participates in receptor interaction and serves a regulatory role via intramolecular interaction with the C-terminal acidic region. Also, two centrally localized domains are directly involved in determining receptor binding specificity and selectivity. (Ref.: Gurevich, VV et al. (1995). J. Biol. Chem. 270 (2); 720-731).

Specificity

This rabbit polyclonal antibody detects Arrestin-C in mouse and rat retina. it targets an epitope with in 12 amino acids from the C-terminal end.

Immunogen

Epitope: C-terminus
Synthetic Linear Peptide

Application

Anti-Cone Arrestin, Cat. No. AB15282, is a highly specific rabbit polyclonal antibody that targets Arrestin-C and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.
Immunohistochemistry Analysis: A 1:250-1,000 dilution from a representative lot detected Cone Arrestin in rat retina, mouse retina, and mouse cerebral cortex tissue.
Research Category
Neuroscience
Research Sub Category
Sensory & PNS

Quality

Evaluated by Western Blotting in mouse retinal tissue lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected Cone Arrestin in 10 µg of mouse retinal tissue lysate.

Target description

~41.9 kDa

Physical form

ImmunoAffinity Purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Quality

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