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Anti-Nicotinic Acetylcholine Receptor α4 Antibody

from rabbit, purified by affinity chromatography

Cholinergic receptor, nicotinic, alpha 4 (neuronal), Cholinergic receptor, nicotinic, alpha polypeptide 4, Neuronal nicotinic acetylcholine receptor alpha-4 subunit, Neuronal acetylcholine receptor subunit alpha-4

biological source


Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies



purified by

affinity chromatography

species reactivity

human, rat, mouse


western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

Gene Information

human ... CHRNA4(1137)

General description

Nicotinic acetylcholine receptors, or nAChRs, are cholinergic receptors that form ligand-gated ion channels in the plasma membranes of certain neurons. nAChR is triggered by the binding of the neurotransmitter acetylcholine (ACh) and is also opened by nicotine. After binding ACh, the nAChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. nAChRs are present in many tissues in the body: the neuronal receptors are present in the central nervous system and the peripheral nervous system; and the neuromuscular receptors are found in the neuromuscular junctions of somatic muscles. Neuronal AChR is composed of two different types of subunits: alpha (α) and beta (β).


This antibody recognizes Nicotinic Acetylcholine Receptor α4.


Epitope: Extracellular Domain
This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from residues of Nicotinic Acetylcholine Receptor α4.


Detect Nicotinic Acetylcholine Receptor α4 using this Anti-Nicotinic Acetylcholine Receptor α4 Antibody validated for use in WB.
Research Category
Research Sub Category
Neurotransmitters & Receptors


Evaluated by Western Blot in rat e16 brain lysate.
Western Blot Analysis: : 1:1,000 dilution of this lot detected Nicotinic Acetylcholine Receptor α4 in 10 µg of e16 rat brain lysate.

Target description

Approx. 55 kDa

Physical form

Antigen Affinity Purified
Rabbit purified polyclonal serum in buffer containing 0.1 M Tris-Glycine (pH7.4) 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Maintain refrigerated at 2-8°C for up to 12 months from date of receipt.

Analysis Note

Rat e16 brain lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Quality

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