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AB15325

Sigma-Aldrich

Anti-Nicotinic Acetylcholine Receptor β2 Antibody

from rabbit, purified by affinity chromatography

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Synonym(s):
Neuronal nicotinic acetylcholine receptor beta 2
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

rat, mouse, human

technique(s)

western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

General description

Nicotinic acetylcholine receptors, also known as nAChRs, belong to a family of proteins called ligand gated ion channels and are widely expressed throughout the nervous system. nAChRs are made up of 5 subunits (two α and three β). Subunits α4 and β2 (α4β2) is the main receptor in the mammalian brain and has been associated with the positive reinforcing effects of nicotine. The relative contribution of nAChRs β2 and β4 are not well understood because the subunit structure that mediates synaptic transmission in the peripheral nervous system is unknown.

Specificity

This antibody recognizes Nicotinic Acetylcholine Receptor β2.

Immunogen

This antibody is generated from rabbits immunized with a KLH-conjugated linear peptide selected from residues of Nicotinic Acetylcholine Receptor β2.

Application

Detect Nicotinic Acetylcholine Receptor β2 using this Anti-Nicotinic Acetylcholine Receptor β2 Antibody validated for use in WB.

Quality

Routinely evaluated by Western Blot in rat e16 brain lysate.
Western Blot Analysis: : 1:20,000 dilution of this lot detected Nicotinic Acetylcholine Receptor β2 on 10 µg of rat e16 brain lysate.

Target description

Approx. 55 kDa

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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Sarah Y Sottile et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 37(47), 11377-11389 (2017-10-25)
Acetylcholine (ACh) is a potent neuromodulator capable of modifying patterns of acoustic information flow. In auditory cortex, cholinergic systems have been shown to increase salience/gain while suppressing extraneous information. However, the mechanism by which cholinergic circuits shape signal processing in
Charles C Lee et al.
Neuroreport, 26(12), 688-694 (2015-07-15)
The thalamocortical pathways form highly topographic connections from the primary sensory thalamic nuclei to the primary cortical areas. The synaptic properties of these thalamocortical connections are modifiable by activation from various neuromodulators, such as acetylcholine. Cholinergic activation can alter functional

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