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AB1576

Sigma-Aldrich

Anti-Cysteine String Protein Antibody

serum, Chemicon®

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Synonym(s):
CSP, DnaJ Homologue Subfamily C member 5
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity (predicted by homology)

all

manufacturer/tradename

Chemicon®

technique(s)

western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... DNAJC5(80331)

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HPA012737AB1543PSAB1406496
vibrant-m

AB1543P

Anti-Synapsin I Antibody

antibody form

serum

antibody form

affinity isolated antibody

antibody form

affinity purified immunoglobulin

antibody form

purified immunoglobulin

biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

mouse

technique(s)

western blot: suitable

technique(s)

immunoblotting: 0.04-0.4 μg/mL, immunohistochemistry: 1:50-1:200

technique(s)

ELISA: suitable, immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: 1 μg/mL

NCBI accession no.

NM_025219.1

NCBI accession no.

-

NCBI accession no.

NM_006950.3

NCBI accession no.

BC003011.1

manufacturer/tradename

Chemicon®

manufacturer/tradename

-

manufacturer/tradename

Chemicon®

manufacturer/tradename

-

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General description

Cysteine-string proteins are membrane-bound proteins associated primarily with nerve endings and synaptic vesicles in the brain where they serve an important role in presynaptic function and may be involved in calcium-dependent neurotransmitter release at nerve endings. CSPs posses a strech of amino acids highly enriched in cysteine residues that are post-translationally palmitoylated. CSPs may function as homodimers.

Specificity

Cysteine-string Protein. The antibody recognizes primarily the alpha isoform of CSP with some recognition of the beta isoforms. Slight reactivity with the gamma isoforms may be detected.

Immunogen

Recombinant rat cysteine-string protein with the cysteine string deleted.

Application

Immunohistochemistry on formalin fixed tissue: 1:1,000. (Recommend Cat. No. AB1576P).Immunocytochemistry on PC-12 cells: 1:1,000. (Recommend Cat. No. AB1576P).Immunoblotting: 1:5,000. CSPs should be resolved on 12-15% SDS-PAGE gels under NON-REDUCING conditions. CSPs typically migrate as 33-35 kDa species in vertebrate samples (dimers may also be seen). If one must perform SDS-PAGE under reducing conditions, it is necessary to alkylate the CSPs to prevent smearing.To ensure proper identification of CSPs, samples may be deacylated by treatment with 1M neutral hydroxylamine or 0.1M KOH in methanol resulting in a 6-7 kDa shift in electrophoretic mobility of the CSP band. Deacylation will usually result imn some loss of reactivity on western blots so sufficient protein should be loaded. Alternatively, because all CSPs appear to be acidic (approximate pI of 4.6-4.8) 2-D gel electrophoresis may be usefull to distinguish some CSPs. Finally, some sample treatments may cause partial deacylation of CSPs resulting in a smear of immunoreactivity below the mass of the fatty acylated monomer.Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Research Sub Category
Synapse & Synaptic Biology
This Anti-Cysteine String Protein Antibody is validated for use in WB, IC, IH for the detection of Cysteine String Protein.

Physical form

Liquid rabbit serum containing no preservatives

Storage and Stability

Store at -20°C in undiluted aliquots for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles.

Analysis Note

Control
POSITIVE CONTROL: brain samples

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Laura Caló et al.
Brain : a journal of neurology, 144(6), 1661-1669 (2021-03-25)
α-Synuclein aggregation at the synapse is an early event in Parkinson's disease and is associated with impaired striatal synaptic function and dopaminergic neuronal death. The cysteine string protein (CSPα) and α-synuclein have partially overlapping roles in maintaining synaptic function and
Evidence that the presynaptic vesicle protein CSPalpha is a key player in synaptic degeneration and protection in Alzheimer's disease.
Tiwari, SS; d'Orange, M; Troakes, C; Shurovi, BN; Engmann, O; Noble, W; Hortobagyi, T; Giese, KP
Molecular Brain null
Huzefa Rupawala et al.
Brain communications, 4(4), fcac192-fcac192 (2022-08-06)
In Alzheimer's disease, synapse loss causes memory and cognitive impairment. However, the mechanisms underlying synaptic degeneration in Alzheimer's disease are not well understood. In the hippocampus, alterations in the level of cysteine string protein alpha, a molecular co-chaperone at the
Bruno A Benitez et al.
Acta neuropathologica communications, 3, 73-73 (2015-11-28)
Autosomal dominant adult-onset neuronal ceroid lipofuscinosis (AD-ANCL) is a multisystem disease caused by mutations in the DNAJC5 gene. DNAJC5 encodes Cysteine String Protein-alpha (CSPα), a putative synaptic protein. AD-ANCL has been traditionally considered a lysosomal storage disease based on the
Ching-Feng Chen et al.
Visual neuroscience, 39, E003-E003 (2022-05-12)
During the first postnatal week in rodents, cholinergic retinal waves initiate in starburst amacrine cells (SACs), propagating to retinal ganglion cells (RGCs) and visual centers, essential for visual circuit refinement. By modulating exocytosis in SACs, dynamic changes in the protein

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