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AB15810

Sigma-Aldrich

Anti-Substance P Receptor Antibody

Chemicon®, from guinea pig

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Synonym(s):
Substance-P receptor, Neurokinin-1 receptor, NK-1 receptor, NK-1R, SPR, Tachykinin receptor 1
eCl@ss:
32160702
NACRES:
NA.41

biological source

guinea pig

Quality Level

antibody form

unpurified

antibody product type

primary antibodies

clone

polyclonal

species reactivity

rat, mouse, human

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry: suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... TACR1(6869)

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This Item
ABN1369S8305AB5060
antibody form

unpurified

antibody form

affinity isolated antibody

antibody form

IgG fraction of antiserum

antibody form

serum

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

species reactivity

rat, mouse, human

species reactivity

rat, human, mouse

species reactivity

human, rat, mouse, guinea pig

species reactivity

rat, guinea pig, mouse

manufacturer/tradename

Chemicon®

manufacturer/tradename

-

manufacturer/tradename

-

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry: suitable, western blot: suitable

technique(s)

immunohistochemistry: suitable, western blot: suitable

technique(s)

immunohistochemistry (frozen sections): 1:5,000 using 4% paraformaldehyde (with picric acid/glutaraldehyde)-fixed, frozen, rat brain sections., microarray: suitable, western blot (chemiluminescent): 1:2,000 using a rat brain membrane fraction extract

technique(s)

immunofluorescence: suitable, immunohistochemistry: suitable, western blot: suitable

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General description

Substance-P receptor (UniProt P14600; also known as Neurokinin-1 receptor, NK-1 receptor, NK-1R, SPR, Tachykinin receptor 1) is encoded by the Tacr1 (also known as Nk1r, Tac1r) gene (Gene ID 24807) in rat species. NK-1, NK-2 and NK-3 are receptors that mediate the biological activities of tachykinins, including substance P (SP), neurokinin A (NKA), neurokinin B (NKB), ranakinin, kassinin, neuropeptide gamma, and eledoisin, widely distributed in the central and peripheral nervous systems. SP shows the highest affinity toward NK-1R (substance-P receptor) and exerts its biological activities via NK-1R, including addition, depression, chronic inflammation, and cancer progression. The involvement of NK-1R in acute pancreatitis through mediating neurogenic inflammation has also been reported. Upon stimulation by SP, the NK-1R interacts with multiple G proteins, including Gαs, Gαq/11, Gαi/o, Gα12, and Gα13, generating second messengers and activating downstream effector molecules. After binding NK-1R, SP induces a clathrin-dependent internalization of the receptor-ligand complex, SP is then degraded and the receptor is recycled to the cell surface. NK-1R is a 7-transmembrane (32-54, 65-86, 107-128, 149-169, 195-219, 249-270, 284-308) GPCR, having its N-terminal end (a.a. 1-31) at the extracelluar side and C-terminal end (a.a. 309-407) at the cytoplasmic side. There exist a second alternatively spliced human NK-1R isoform that lacks the C-terminal cytoplasmic domain (a.a. 312-407 missing). The full-length form enhances the growth of cancer cells and stimulates the production of cytokines, which up-regulate the truncated form. The truncated form mediates malignancy and is more abundant in colonic epithelial cells from patients with colitis-associated cancer. In cancer cells, the NK-1 receptor is mainly localized on the plasma membrane and in cytoplasm, and occasionally also in the nucleus.

Specificity

This guinea pig polyclonal antiserum targets substance P receptor C-terminal end sequence. Target region is absent in human spliced isoform 2.

Immunogen

Epitope: C-Terminus
KLH-conjugated linear peptide corresponding to the C-terminal end sequence of rat substance P receptor.

Application

Research Category
Neuroscience
Research Sub Category
Synapse & Synaptic Biology
This Guinea pig polyclonal Anti-Substance P Receptor Antibody, Cat. No. AB15810 detects levels of Substance P receptor, and has been published and validated for use in Immunohistochemistry, and Western Blotting.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected substance P receptor in 10 µg of human brain tissue lysate.

Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected substance P receptor in rat brain tissue sections.

Quality

Evaluated by Western Blotting in rat brain tissue lysate.

Western Blotting Analysis: A 1:5000 dilution from a representative lot detected substance-P receptor in 10 µg of rat brain tissue lysate.

Target description

~58/54 kDa observed. Target band size appears larger than the calculated molecular weights of 46.25/46.32/46.37 kDa (human/mouse/rat) due to glycosylation. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Unpurified
Guinea Pig polyclonal antibody serum with 0.05% sodium azide
Unpurified.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
Rat brain tissue lysate

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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BMC Physiology null
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