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AB1603

Sigma-Aldrich

Anti-Phosphoserine Antibody

Chemicon®, from rabbit

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eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity (predicted by homology)

all

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

shipped in

wet ice

target post-translational modification

phosphorylation (pSer)

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This Item
AB1607ABS1670SAB5200086
antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

manufacturer/tradename

-

manufacturer/tradename

-

technique(s)

ELISA: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

ELISA: suitable, immunohistochemistry: suitable (paraffin), immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

ELISA: suitable, dot blot: suitable, immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

immunohistochemistry: suitable, immunoprecipitation (IP): suitable, indirect ELISA: suitable, western blot: suitable

shipped in

wet ice

shipped in

wet ice

shipped in

ambient

shipped in

wet ice

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General description

Cellular responses to a variety of extracellular signals occur through phosphorylation or dephosphorylation of intracellular proteins. Abnormal protein phosphorylation may be involved in the progression of numerous diseases, including many forms of cancer, immune system dysfunction and cardiovascular disease. Such changes in protein phosphorylation may be detected by immunocytochemical mapping of cells labeled with anti-phosphoserine or anti-phosphothreonine antibodies and can be correlated with cellular, electrophysiological or behavioral state changes.

Specificity

Anti-phosphoserine is species independent.
Recognizes phosphoserine, peptidyl-phosphoserine, and serine-phosphorylated proteins. Does not cross react with ATP, phosphotyrosine, peptidyl phosphothreonine and serine. Slight cross reactivity with free phosphothreonine. Readily reacts with known phosphoproteins such as phosvitin and alpha casein. Anti-phosphoserine is species independent in reactivity

Immunogen

Keyhole Limpet Hemocyanin(KLH)- conjugated phosphoserine conjugates.

Application

Anti-Phosphoserine Antibody detects level of Phosphoserine & has been published & validated for use in ELISA, IH, IP & WB.
Immunoprecipitation (tissue extracts):
10-20 μg anti-pS/mg protein was used from a previous lot.
Note that this will immunoprecipitate all phosphoserine proteins in the extracts. If using purified protein exacts containing only the protein of interest, antibody amounts should be decreased to 5 μg/500 μL of extracts. Optimal concentrations must be determined experimentally.

ELISA (kinase assay):
A previous lot of this anitbody was used at a 1:250-1:500 dilution.

Immunohistochemistry:
A previous lot of this antibody was used at a 1:50 dilution.

As the antibody detects all phosphoserines, any phosphorylated serine protein or peptide can be used to block antibody staining.
Typically a 10 M excess of peptide is used in Tris based buffers.

Western Blot Analysis:
1:500 dilution of a previous lot

Optimal working dilutions must be determined by end user.
Research Category
Signaling
Research Sub Category
General Post-translation Modification

Quality

Routinely evaluated by Western Blot on NGF treated PC12 lysates.

Western Blot Analysis: 1:500 dilution of this lot detected serine phosphorylated proteins on 10 μg of NGF treated PC12 lysates.

Target description

Dependent upon the molecular weight of the serine phosphorylated protein being detected.

Physical form

Phosphoserine affinity chromatography
Purified rabbit serum in buffer containing 50% glycerol.

Storage and Stability

Stable for up to 6 months at -20°C in undiluted aliquots from date of receipt. During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 uL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container′s cap.

Analysis Note

Control
NIH 3T3 cells (+/- TPA), K562 cells, and EGF-stimulated A431 cells.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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A novel high throughput biochemical assay to evaluate the HuR protein-RNA complex formation.
D'Agostino, VG; Adami, V; Provenzani, A
Testing null
A Crohn's disease-associated NOD2 mutation suppresses transcription of human IL10 by inhibiting activity of the nuclear ribonucleoprotein hnRNP-A1.
Noguchi, E; Homma, Y; Kang, X; Netea, MG; Ma, X
Nature Immunology null
Bon-Hun Koo et al.
The Journal of biological chemistry, 287(27), 22643-22653 (2012-05-12)
Matrix metalloproteinase-2 (MMP-2) functions in diverse biological processes through the degradation of extracellular and non-extracellular matrix molecules. Because of its potential for tissue damage, there are several ways to regulate MMP-2 activity, including gene expression, compartmentalization, zymogen activation, and enzyme
Pkd2+/- vascular smooth muscles develop exaggerated vasocontraction in response to phenylephrine stimulation.
Qian, Q; Hunter, LW; Du, H; Ren, Q; Han, Y; Sieck, GC
Journal of the American Society of Nephrology null
Liangzhen Jiang et al.
PloS one, 8(8), e72289-e72289 (2013-08-27)
Polo-like kinase 1 (Plk1) is a highly conserved Ser/Thr kinase in eukaryotes and plays a critical role in various aspects of the cell cycle. Plk1 exerts its multiple functions by phosphorylating its substrates. In this study, we found that Plk1

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