AB1620

Anti-Vimentin Antibody

serum, Chemicon^®

• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: goat
• Quality Level: 100
• antibody form: serum
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: human
• species reactivity (predicted by homology): mammals
• manufacturer/tradename: Chemicon^®
• technique(s): immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• NCBI accession no.: NM_003380.2
• UniProt accession no.: P08670
• shipped in: dry ice
• Gene Information: human ... VIM(7431)

Description

Specificity

Recognizes vimentin in cultured mammalian cells and tissue sections. AB1620 specifically stains the vimentin band (58 kD) using total cell or tissue extracts in immunoblotting. No reactivity to desmin, keratin, neurofilaments or glial filaments. Shows wide cross-reactivity among mammalian species using various immunolabeling techniques.

Immunogen

Vimentin purified from cultured human foreskin fibroblasts.

Application

Anti-Vimentin Antibody detects level of Vimentin & has been published & validated for use in IC, IH, IH(P) & WB.

Immunohistochemistry: 1:20-1:40. Shows reactivity on frozen tissue sections as well as formalin-fixed paraffin embedded tissue by immunoperoxidase and immunofluorescent assays.

Immunocytochemistry

Western blot

Optimal working dilutions must be determined by end user.

Research Category
Cell Structure

Research Sub Category
Cytoskeleton

Target description

58 kDa

Physical form

Neat goat Serum. Liquid containing 0.1% sodium azide.

Unpurified

Storage and Stability

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Breast carcinoma tissue, Colon, Prostate, Tonsil, Tonsilar lymphoma, Uterus, Ureter

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable