All Photos(1)

AB2033

Sigma-Aldrich

Anti-Fibronectin Antibody

Chemicon®, from rabbit

eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse

manufacturer/tradename

Chemicon®

application(s)

ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
radioimmunoassay: suitable

NCBI accession no.

UniProt accession no.

shipped in

dry ice

General description

Fibronectin is a high molecular weight extracellular adhesion molecule involved in many cellular processes, including tissue repair or wound healing, embryogenesis, blood clotting, and cell migration/adhesion. Fibronectin exists in two major forms: 1) as an insoluble glycoprotein dimer that serves as a linker in the extracellular matrix (ECM), and 2) as a soluble disulfide-linked dimer found in the plasma. The plasma form is synthesized by hepatocytes, and the ECM form is made by fibroblasts, chondrocytes, endothelial cells, macrophages, as well as certain epithelial cells. Fibronectin sometimes serves as a general cell adhesion molecule by anchoring cells to collagen or proteoglycan substrates. It can also serve to organize cellular interaction with the ECM by binding to different components of the ECM and to membrane-bound fibronectin receptors on cell surfaces. The immunogen for fibronectin polyclonal antibody AB2033 is purified mouse plasma. The antibody shows less than 0.1% reactivity with mouse laminin, and collagen types I, III, and IV by radioimmunoassay.

Specificity

This antibody reacts with mouse fibronectin. Shows less than 0.1% reactivity with mouse Laminin, and Collagen Types I, III, and IV by radioimmunoassay.

Immunogen

Fibronectin extracted and purified from mouse plasma.

Application

Research Category
Cell Structure
Immunohistochemistry:
1:80 dilution of a previous lot was used in immunofluorescent staining of frozen mouse skin and liver tissues.

Radioimmunoassay:
A previous lot of this antibody was used in RIA.

ELISA:
A 1:200 dilution of a previous lot was used in ELISA.

Optimal working dilutions must be determined by the end user.
Detect Fibronectin using this Anti-Fibronectin Antibody validated for use in ELISA, IC, IH, RIA.
Research Sub Category
ECM Proteins

Quality

Evaluated by immunocytochemistry on NIH/3T3 cells.

Immunocytochemistry:
Representative image from a previous lot.
Confocal fluorescent analysis of NIH/3T3 using AB2033 rabbit polyclonal antibody (Red). Actin filaments have been labeled with AlexaFluor 488 -Phalloidin (Green). Nucleus is stained with DAPI (Blue). Positive staining is restricted to the cytosol.

Physical form

ImmunoAffinity Purified
Purified rabbit polyclonal in buffer containing 0.01 M phosphate, 0.09 M NaCl, pH 7.2 with no preservatives.

Storage and Stability

Stable for 1 year at -20ºC from date of receipt.

Analysis Note

Control
Extracellular matrix in most tissue

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Cutting Edge: mechanical forces acting on T cells immobilized via the TCR complex can trigger TCR signaling.
Li YC, Chen BM, Wu PC, Cheng TL, Kao LS, Tao MH, Lieber A, Roffler SR
Journal of immunology (Baltimore, Md. : 1950) null
Plasma fibronectin is neuroprotective following traumatic brain injury.
Ciara C Tate, Andres J Garcia, Michelle C LaPlaca
Experimental neurology null
Raquel Rodrigues-Díez et al.
Kidney international, 86(2), 303-315 (2014-02-21)
The classical view of the immune system has changed by the discovery of novel T-helper (Th) subsets, including Th17 (IL-17A-producing cells). IL-17A participates in immune-mediated glomerulonephritis and more recently in inflammatory pathologies, including experimental renal injury. Peritoneal dialysis patients present...
Atrial natriuretic peptide-dependent modulation of hypoxia-induced pulmonary vascular remodeling.
Yiu-Fai Chen, Ji-An Feng, Peng Li, Dongqi Xing, Namasivayam Ambalavanan, Suzanne Oparil
Life Sciences null
Katsutoshi Sekine et al.
Neuron, 76(2), 353-369 (2012-10-23)
Birthdate-dependent neuronal layering is fundamental to neocortical functions. The extracellular protein Reelin is essential for the establishment of the eventual neuronal alignments. Although this Reelin-dependent neuronal layering is mainly established by the final neuronal migration step called "terminal translocation" beneath...

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