MilliporeSigma
  • AB2253
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AB2253

Sigma-Aldrich

Anti-Doublecortin Antibody

serum, from guinea pig

Synonym(s):
Doublin, Lissencephalin-X, Lis-X
eCl@ss:
32160702
NACRES:
NA.41

biological source

guinea pig

Quality Level

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

mol wt

(Target molecular weight ~42 kDa observed;40.61 kDa calculated. Uncharacterized bands may be observed in some lysate(s).)

species reactivity

rat, mouse

species reactivity (predicted by homology)

human (based on 100% sequence homology)

technique(s)

immunofluorescence: suitable
immunohistochemistry: suitable ((Paraffin))
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

Gene Information

human ... DCDC1(341019)

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Sigma-Aldrich

ABS500

Anti-CDT2 Antibody

biological source

guinea pig

biological source

rabbit

biological source

rabbit

biological source

rabbit

antibody form

serum

antibody form

serum

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

mol wt

(Target molecular weight ~42 kDa observed;40.61 kDa calculated. Uncharacterized bands may be observed in some lysate(s).)

mol wt

-

mol wt

antigen ~40 kDa

mol wt

antigen 44 kDa

species reactivity

rat, mouse

species reactivity

mouse, rat, human

species reactivity

human, mouse, rat

species reactivity

rat, human, mouse

technique(s)

immunofluorescence: suitable, immunohistochemistry: suitable ((Paraffin)), western blot: suitable

technique(s)

western blot: suitable

technique(s)

western blot: 1-2 μg/mL using HEK-293T cells expressing human doublecortin. Also, mouse and rat brain extract (S1 fraction).

technique(s)

ELISA: 1:10000, western blot: 1:500-1:1000

General description

Anti-Doublecortin, Cat. No. AB2253, is a guinea pig polyclonal antibody that detects Doublecortin and is tested for use in Immunohistochemistry (Paraffin) and Immunofluorescence, and Western Blotting.

Specificity

Reactivity with other species has not been determined.
This Guinea pig polyclonal antibody detects doublecortin in murine and rat species. It targets an epitope with in 17 amino acids from the C-terminal region.

Immunogen

Epitope: C-Terminus
Recombinant Peptide.

Application

Anti-Doublecortin Antibody has been used in immunohistochemistry and immunofluorescence staining.

Quality

Evaluated by Western Blotting in rat brain tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Doublecortin in rat brain tissue lysate.

Target description

~45 kDa observed; 40.57 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Linkage

Replaces: AB5910

Physical form

GST Depletion
GST-Depleted Guinea Pig Sera with 0.05% Sodium Azide

Storage and Stability

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
Rat Brain lysate.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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