All Photos(5)

AB2253

Sigma-Aldrich

Anti-Doublecortin Antibody

serum, from guinea pig

Synonym(s):
Doublin, Lissencephalin-X, Lis-X
eCl@ss:
32160702
NACRES:
NA.41

Quality Level

biological source

guinea pig

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

mouse, rat

species reactivity (predicted by homology)

human

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

Specificity

Reactivity with other species has not been determined.
This Guinea pig polyclonal antibody detects doublecortin in murine and rat species. It targets an epitope with in 17 amino acids from the C-terminal region.

Immunogen

Recombinant Peptide.
Epitope: C-Terminus

Application

Anti-Doublecortin Antibody has been used in immunohistochemistry and immunofluorescence staining.

Quality

Evaluated by Western Blotting in rat brain tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Doublecortin in rat brain tissue lysate.

Target description

~45 kDa observed; 40.57 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Linkage

Replaces: AB5910

Physical form

GST-Depleted Sera with 0.05% NaN3 .
GST Depletion

Storage and Stability

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
Rat Brain lysate.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Embryonic Nkx2. 1-expressing neural precursor cells contribute to the regional heterogeneity of adult V-SVZ neural stem cells
Delgado RN and Lim DA
Developmental Biology, 407(2), 265-274 (2015)
Adolescent Changes in Cellular Proliferation in the Dentate Gyrus of Male and Female C57BL/6N Mice Are Resilient to Chronic Oral Corticosterone Treatments
Shome A, et al.
Frontiers in Behavioral Neuroscience, 12, 192-192 (2018)
Tania Campos-Ordoñez et al.
Experimental neurology, 261, 236-244 (2014-05-27)
Hydrocephalus can develop secondarily to a disturbance in production, flow and/or absorption of cerebrospinal fluid. Experimental models of hydrocephalus, especially subacute and chronic hydrocephalus, are few and limited, and the effects of hydrocephalus on the subventricular zone are unclear. The
Combination treatment with ethyl pyruvate and IGF-I exerts neuroprotective effects against brain injury in a rat model of neonatal hypoxic-ischemic encephalopathy
Rong Z, et al.
International Journal of Molecular Medicine, 36(1), 195-203 (2015)
Tomás P Bachor et al.
Neurobiology of disease, 108, 13-28 (2017-07-27)
Diabetes mellitus (DM) is reaching epidemic conditions worldwide and increases the risk for cognition impairment and dementia. Here, we postulated that progenitors in adult neurogenic niches might be particularly vulnerable. Therefore, we evaluated the different components of the mouse subventricular

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service