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AB2301

Sigma-Aldrich

Anti-Calnexin Antibody

from rabbit, purified by affinity chromatography

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Synonym(s):
Calnexin, IP90, Major histocompatibility complex class I antigen-binding protein p88, p90
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse, rat, human, horse, pig

species reactivity (predicted by homology)

equine (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology), chimpanzee (based on 100% sequence homology), bovine (based on 100% sequence homology), porcine (based on 100% sequence homology)

packaging

antibody small pack of 25 μL

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... CANX(821)

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This Item
SAB4503258C7617C4731
antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

antibody form

IgG fraction of antiserum

clone

polyclonal

clone

polyclonal

clone

TO-5, monoclonal

clone

polyclonal

species reactivity

mouse, rat, human, horse, pig

species reactivity

mouse, rat, human

species reactivity

human

species reactivity

canine, rat, mouse, human

packaging

antibody small pack of 25 μL

packaging

-

packaging

-

packaging

antibody small pack of 25 μL

technique(s)

immunocytochemistry: suitable, immunohistochemistry: suitable (paraffin), western blot: suitable

technique(s)

ELISA: 1:1000, immunofluorescence: 1:100-1:500, immunohistochemistry: 1:50-1:100, western blot: 1:500-1:1000

technique(s)

flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, indirect ELISA: suitable, western blot: 0.5-1.0 μg/mL using total cell extract of HeLa cells.

technique(s)

immunoprecipitation (IP): 1:2,000 using whole cell RIPA lysate of the human epitheloid carcinoma HeLa cell line, indirect immunofluorescence: 1:200 using 3% paraformaldehyde-fixed, 0.5% Triton X-100 treated, Madin Darby canine kidney MDCK cell line, microarray: suitable, western blot: 1:2,000 using whole cell RIPA lysate of the human hepatocytoma HepG2 cell line

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General description

Calnexin is a Ca++ binding protein found in ER transmembranes that binds to specific n-linked oligosaccharides found on newly synthesized proteins. Calnexin associates with the ER membrane phosphoglycoprotein, signal sequence receptor SSRμ, and has been identified as the ER protein associated with major histocompatibility complex (MHC) class I heavy chain and with the T-cell receptor and membrane immunoglobulin before assembly of these multimeric complexes. Hence, calnexin may represent a molecular chaperone in the ER membrane that is selective for incompletely folded oligomeric membrane proteins newly translocated to the ER.

Specificity

This antibody recognizes Calnexin at the C-terminus.

Immunogen

Epitope: C-terminus
KLH-conjugated linear peptide corresponding to the C-terminus of human Calnexin.

Application

Anti-Calnexin Antibody is an antibody against Calnexin for use in WB, IC, IH(P).
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Calnexin in NIH/3T3 cells.

Immunohistochemistry Analysis: A 1:1,000-2,000 dilution from a representative lot detected Calnexin in human brain and human prostate tissues.
Research Category
Neuroscience
Research Sub Category
Developmental Neuroscience

Quality

Evaluated by Western Blot in human brain tissue lysate.

Western Blot Analysis: A 1:10,000 dilution of this antibody detected Calnexin on 10 µg of human brain tissue lysate.

Target description

~70 kDa observed

Physical form

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Human brain tissue lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Caroline M Gorvin et al.
Human molecular genetics, 27(21), 3720-3733 (2018-07-28)
The calcium-sensing receptor (CaSR) is a homodimeric G-protein-coupled receptor that signals via intracellular calcium (Ca2+i) mobilisation and phosphorylation of extracellular signal-regulated kinase 1/2 (ERK) to regulate extracellular calcium (Ca2+e) homeostasis. The central importance of the CaSR in Ca2+e homeostasis has
Caroline M Gorvin et al.
Science signaling, 11(518) (2018-02-22)
The calcium-sensing receptor (CaSR) is a G protein-coupled receptor (GPCR) that signals through Gq/11 and Gi/o to stimulate cytosolic calcium (Ca2+i) and mitogen-activated protein kinase (MAPK) signaling to control extracellular calcium homeostasis. Studies of loss- and gain-of-function CASR mutations, which
Shuhiba Mohammad et al.
Scientific reports, 11(1), 12615-12615 (2021-06-18)
The physiological and molecular mechanisms linking prenatal physical activity and improvements in maternal-fetal health are unknown. It is hypothesized that small extracellular vesicles (EVs, ~ 10-120 nm) are involved in tissue cross-talk during exercise. We aimed to characterize the circulating small EV profile
Carly N Logan et al.
Psychopharmacology, 237(7), 2007-2018 (2020-05-10)
The beta-lactam antibiotic ceftriaxone reliably attenuates the reinstatement of cocaine seeking. While the restoration of nucleus accumbens core (NA core) GLT-1 expression is necessary for ceftriaxone to attenuate reinstatement, AAV-mediated GLT-1 overexpression is not sufficient to attenuate reinstatement and does
Carly N Logan et al.
Neuropharmacology, 135, 297-307 (2018-03-24)
Cocaine use disorder is a chronically relapsing disease without FDA-approved treatments. Using a rodent model of cocaine relapse, we and others have previously demonstrated that the beta-lactam antibiotic ceftriaxone attenuates cue- and cocaine-primed reinstatement of cocaine-seeking. Ceftriaxone restores cocaine-induced deficits

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