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  • AB3152
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AB3152

Sigma-Aldrich

Anti-Amphiregulin Antibody

Chemicon®, from rabbit

Synonym(s):
AR, CRDGF, KAF
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human, mouse

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunoprecipitation (IP): suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

Gene Information

human ... AREG(374)

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This Item
07-35007-363171372
biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

-

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

-

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

-

species reactivity

human, mouse

species reactivity

human

species reactivity

human, rat

species reactivity

-

manufacturer/tradename

Chemicon®

manufacturer/tradename

Upstate®

manufacturer/tradename

Upstate®

manufacturer/tradename

Calbiochem®

technique(s)

ELISA: suitable, immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

immunohistochemistry: suitable, western blot: suitable

technique(s)

western blot: suitable

technique(s)

-

Specificity

Recognizes a glycoprotein of 18.5-22.5kDa, identified as amphiregulin (AR). Antibody is directed against the region of AR, which binds to heparin and is highly specific to AR. It shows no cross-reaction with EGF, TGFalpha, or HB-EGF. In vitro, AR functions as an autocrine growth stimulator to derive proliferation of colon carcinoma cells, normal and oncogene-transformed mammary epithelial cells, cervical carcinoma cells, prostate cancer cells, and keratinocytes. AR is important in development of human placenta and murine mammary gland.

Immunogen

A synthetic 19-mer peptide, corresponding to aa 26-44 from the secreted amphiregulin of human origin.

Application

Immunoprecipitation (Use Protein A): Ab 10μg/mg protein lysate.

Western Blotting: Ab 5μg/ml for 2hrs at room temperature.

Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Research Sub Category
Neurochemistry & Neurotrophins
This Anti-Amphiregulin Antibody is validated for use in ELISA, IP, WB for the detection of Amphiregulin.

Physical form

Format: Purified
Purified immunoglobulin from rabbit anti-serum by Protein A chromatography. Liquid in 10mM PBS, pH 7.4, with 0.2% BSA and 15mM sodium azide.

Storage and Stability

Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Please enter the Product Number and Lot Number.

Certificate of Quality

Please enter the Product Number and Lot Number.

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