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AB3201

Sigma-Aldrich

Anti-Tubulin Antibody, Detyrosinated

Chemicon®, from rabbit

Synonym(s):
Glu Tubulin
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

rat, human, sea urchin, plant, mouse

should not react with

Saccharomyces cerevisiae

species reactivity (predicted by homology)

mammals

manufacturer/tradename

Chemicon®

application(s)

immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

dry ice

General description

Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein which consists of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter. Tyrosine ligase ads a C-terminal tyrosin to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation which is characteristic for microtubules in neuronal cells and the mitotic spindle.

Specificity

Expected to cross-react with mammals, sea urchin, plants, not yeast (Paturle-Lafanechere, L., 1991).
The antibody specifically recognizes the detyrosinated form (Glu tubulin) of the tubulin alpha chain (Bianchi, M., 2006; Goulimari, P., 2005).

Immunogen

Peptide corresponding to the C-terminal of Glu tubulin.

Application

Research Category
Cell Structure
Research Sub Category
Cytoskeletal Signaling
Anti-Tubulin Antibody, Detyrosinated is an antibody against Tubulin for use in IC, IH(P) and WB.
Immunohistochemistry:
A previous lot was used on either frozen or paraffin embedded, fixed tissue (Gundersen, G.G., 1984): 1:200-1:500 dilution.

Optimal working dilutions must be determined by the end user.

Quality

Routinely evaluated by Western Blot on L6 lysate lysates.

Western Blot Analysis: 1:500 dilution of this lot detected detyrosinated tubulin on 10 μg of L6 lysate.

Target description

50 kDa

Physical form

Affinity purified immunoglobulin
Purified rabbit polyclonal in buffer containing liquid PBS.

Storage and Stability

Stable for 6 months at -20°C in undiluted aliquots from date of receipt.

Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
Hela Whole Cell Lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Geraldine B Quinones et al.
Molecular biology of the cell, 22(7), 1045-1057 (2011-02-11)
Tubulin posttranslational modifications (PTMs) have been suggested to provide navigational cues for molecular motors to deliver cargo to spatially segregated subcellular domains, but the molecular details of this process remain unclear. Here we show that in Madin-Darby Canine Kidney (MDCK)
Histone deacetylase 6 inhibition compensates for the transport deficit in Huntington's disease by increasing tubulin acetylation.
Dompierre, JP; Godin, JD; Charrin, BC; Cordelieres, FP; King, SJ; Humbert, S; Saudou, F
The Journal of Neuroscience null
Posttranslational nitrotyrosination of alpha-tubulin induces cell cycle arrest and inhibits proliferation of vascular smooth muscle cells.
Anh D Phung, Karel Soucek, Lukas Kubala, Richart W Harper, J Chloe Bulinski, Jason P Eiserich
European Journal of Cell Biology null
Isolation rearing induces recognition memory deficits accompanied by cytoskeletal alterations in rat hippocampus.
Bianchi, M, et al.
The European Journal of Neuroscience, 24, 2894-2902 (2006)
Infection with replication-deficient adenovirus induces changes in the dynamic instability of host cell microtubules.
Warren, JC; Rutkowski, A; Cassimeris, L
Molecular Biology of the Cell null

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