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AB3442

Sigma-Aldrich

Anti-CREB Antibody, pSer133

serum, Chemicon®

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Synonym(s):
CREB-1, cAMP-response Element-binding Protein-1
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

mouse, human, monkey, rat

manufacturer/tradename

Chemicon®

technique(s)

western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... CREB1(1385)

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AB3006MAB5432AV31264
vibrant-m

AB3442

Anti-CREB Antibody, pSer133

vibrant-m

AB3006

Anti-CREB Antibody

vibrant-m

MAB5432

Anti-CREB Antibody

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

antibody form

serum

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

IgG fraction of antiserum

technique(s)

western blot: suitable

technique(s)

ELISA: suitable, electrophoretic mobility shift assay: suitable, immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

ELISA: suitable, western blot: suitable

technique(s)

western blot: suitable

clone

polyclonal

clone

polyclonal

clone

monoclonal

clone

polyclonal

species reactivity

mouse, human, monkey, rat

species reactivity

human, mouse, rat

species reactivity

human, mouse, rat

species reactivity

mouse, horse, rabbit, rat, dog, human

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Specificity

Recognizes phosphorylated Serine133 in cAMP response element binding protein (CREB). By immunoblotting the antibody shows a 5-10 fold increase in staining of a 43 kDa protein after treatment of cells with agents that increase intracellular cAMP. The antibody also recognizes phosphorylated ATF-1 due to a common epitope. The antibody has been preabsorbed with unphosphorylated peptide and will prove to be useful for the investigation of CREB phosphorylation.

Immunogen

Epitope: pSer133
Phosphopeptide conjugated to KLH.

application

Anti-CREB Antibody, pSer133 is a Rabbit Polyclonal Antibody for detection of CREB also known as CREB-1, cAMP-response Element-binding Protein-1 & has been validated in WB.
Immunoblotting: 1:500-1:1,000

Optimal working dilutions must be determined by the end user.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors

RNA Binding Protein (RBP)

Physical form

Rabbit serum. Liquid. The antibody has been preabsorbed with unphosphorylated peptide. Contains 0.01% sodium azide.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze-thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1


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Jonathan Aryeh Sobel et al.
PLoS biology, 15(4), e2001069-e2001069 (2017-04-18)
Many organisms exhibit temporal rhythms in gene expression that propel diurnal cycles in physiology. In the liver of mammals, these rhythms are controlled by transcription-translation feedback loops of the core circadian clock and by feeding-fasting cycles. To better understand the
Ying Yu et al.
Epilepsia open, 5(3), 418-431 (2020-09-12)
The objective of this study was to identify the signaling pathway that is immediately triggered by status epilepticus (SE) and in turn contributes to the excessive brain-derived neurotrophic factor (BDNF)/tropomyosin-related kinase receptor B (TrkB) signaling within the hippocampus. We used

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