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AB356480

Sigma-Aldrich

Anti-Cas9 (Rabbit Polyclonal)

serum, from rabbit

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Synonym(s):
SaCas9, CRISPR-associated endonuclease Cas9
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

Staphylococcus aureus, bacteria

packaging

antibody small pack of 25 μL

technique(s)

immunofluorescence: suitable
western blot: suitable

isotype

IgG

UniProt accession no.

shipped in

ambient

target post-translational modification

unmodified

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This Item
SAB4200751ABS2202MAC142
Anti-Cas9 (Rabbit Polyclonal) serum, from rabbit

AB356480

Anti-Cas9 (Rabbit Polyclonal)

Anti-Cas9 from rabbit, purified by affinity chromatography

ABS2202

Anti-Cas9

biological source

rabbit

biological source

-

biological source

rabbit

biological source

mouse

antibody form

serum

antibody form

purified from hybridoma cell culture

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

species reactivity

Staphylococcus aureus, bacteria

species reactivity

-

species reactivity

Streptococcus pyogenes, bacteria

species reactivity

Staphylococcus aureus, bacteria

shipped in

ambient

shipped in

dry ice

shipped in

ambient

shipped in

ambient

clone

polyclonal

clone

10C11-A12, monoclonal

clone

polyclonal

clone

6H4, monoclonal

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General description

CRISPR-associated endonuclease Cas9 (UniProt: J7RUA5; also known as SaCas9, Cas9) is encoded by the Cas9 gene in Streptococcus aureus. Cas9 belongs to subtype II-A subfamily with one HNH Cas9-type domain. CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. Cas9 has a bi-lobed architecture with a recognition lobe (residues 41-425) and a discontinuous nuclease lobe (NUC, residues 1-40 and 453-1053); the crRNA-target DNA lies in a channel between the 2 lobes. The NUC lobe has 2 endonuclease domains. The discontinuous RuvC-like domain in NUC cleaves the target DNA non-complementary to crRNA while the HNH nuclease domain cleaves the target DNA complementary to crRNA. Cas9 serves as a RNA-guided DNA endonuclease that cleaves double-stranded DNA targets with a protospacer adjacent motif (PAM) and complementarity to the guide RNA. The homologous region of the Streptococcus pyogenes is not closely related in amino acid sequence in S. aureus, hence this antibody does not recognize that protein.

Specificity

This rabbit polyclonal antibody detects Cas9 from Staphylococcus aureus. It targets an epitope within 251 amino acids from the C-terminal end. It does not react with Cas9 from Streptococcus pyogenes.

Application

Anti-Cas9 (Rabbit Polyclonal), Cat. No. AB356480, is a highly specific rabbit polyclonal antibody that targets CRISPR-associated endonuclease Cas9 and has been tested in Immunofluorescence and Western Blotting.
Immunofluorescence Analysis: A 1:1,000 dilution from a representative lot detected Cas9 in HEK293 cells with transfected with GFP-Cas9-SA (Courtesy of Dr. Gerry Shaw).

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Cas9 in HEK293 cells overexpressing fusion protein GFP-Cas9 (Courtesy of Dr. Gerry Shaw).

Quality

Evaluated by Western Blotting in HEK293 cells overexpressing fusion protein GFP-Cas9.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected Cas9 (Rabbit Polyclonal) in 40 µg of HEK293 cells overexpressing fusion protein GFP-Cas9 from Staphylococcus aureus.

Target description

~55 kDa observed. Uncharacterized bands may be observed in some lysate(s).

Other Notes

Concentration: Please refer to lot specific datasheet.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Zuriah Meacham et al.
PLoS biology, 21(12), e3002431-e3002431 (2023-12-08)
Bacteriophages encode anti-CRISPR (Acr) proteins that inactivate CRISPR-Cas bacterial immune systems, allowing successful invasion, replication, and prophage integration. Acr proteins inhibit CRISPR-Cas systems using a wide variety of mechanisms. AcrIIA1 is encoded by numerous phages and plasmids, binds specifically to

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