AB3623
Anti-Caspase 3 Antibody, active (cleaved) form
Chemicon®, from rabbit
Synonym(s):
Apopain, Yama Protein, CASP3, SCA-1, procaspase3
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50 μG
$526.00
$526.00
Available to ship onMay 15, 2025
A recombinant, preservative-free antibody is available for your target. Try ZRB1221
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50 μG
$526.00
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
$526.00
Available to ship onMay 15, 2025
A recombinant, preservative-free antibody is available for your target. Try ZRB1221
Request a Bulk Order
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
mouse, rat, human
manufacturer/tradename
Chemicon®
technique(s)
immunofluorescence: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... CASP3(836)
General description
Caspase-3 (CPP32, Apopain) is the most extensively studied apoptotic protein. Caspase-3 is synthesized as an inactive proenzyme (32 kDa) that is processed in cells undergoing apoptosis by self-proteolysis and/or cleavage by another upstream protease. The processed form of caspase-3 consists of large (17 kDa) and small (12 kDa) subunits which associate to form an active enzyme. The active caspase-3 proteolytically cleaves and activates other caspases, as well as relevant targets in the cells such as PARP and DFF.
Specificity
Anti-Caspase 3, active (cleaved) is a rabbitpolyclonal antibody that detects active Caspase 3. It targets an epitope within13 amino acids from the C-terminal region.
Immunogen
KLH-conjugated linear peptide corresponding to 13 amino acids from theC-terminal region of human active Caspase 3.
Application
Anti-Caspase 3 Antibody, active (cleaved) form detects level of Caspase 3 and has been published and validated for use in Immunofluorescence (IF), Immunohistochemistry (IHC), and Western Blot (WB).
Quality Control Testing
Evaluated by Western Blotting in Jurkat cells treatedwith Camptothecin. Western Blotting Analysis (WB): A 1:500 dilution from a representative lot detectedactive Caspase-3 in lysate from Jurkat cells treated with Camptothecin (1 μM; 3 hours).
Evaluated by Western Blotting in Jurkat cells treatedwith Camptothecin. Western Blotting Analysis (WB): A 1:500 dilution from a representative lot detectedactive Caspase-3 in lysate from Jurkat cells treated with Camptothecin (1 μM; 3 hours).
Tested Applications Immunohistochemistry (Paraffin) Analysis (IHC): A 1:200dilution from a representative lot detected Caspase 3, active (cleaved) inhuman tonsil and human colon tissuesections. Immunocytochemistry Analysis (ICC): A 1:500 dilution froma representative lot detected active Caspase-3 in HeLa cells treated withStaurosporine (0.5 μM for 6 hours.). Immunocytochemistry Analysis (ICC): A 1:500 dilution froma representative lot detected active Caspase-3 in HeLa cells treated withCamptothecin (3 μM for 6 hours.). Peptide Inhibition Assay (PIA): Target band detection inlysate from Jurkat cells treated with Camptothecin (1μM; 3 hours)was prevented by pre-blocking of a representative lot with the immunogenpeptide. Note: Actual optimal working dilutions must bedetermined by end user as specimens, and experimental conditions may vary withthe end user.
Target description
Target molecular weight ~17 kDa observed. 17kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Linkage
Replaces: 04-440; 04-1088
Physical form
Purified rabbit polyclonal antibody in PBS with 0.05% sodium azide with 30% glycerol.
Storage and Stability
Store at -10°C to -25°C. Handling Recommendations: Upon receipt andprior to removing the cap, centrifuge the vial and gently mix the solution.Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thawcycles, which may damage IgG and affect product performance.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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