AB3786

Anti-Prosurfactant Protein C (proSP-C) Antibody

serum, Chemicon^®

• Synonym(s): proSP-C
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: serum
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: mouse, human, rat
• manufacturer/tradename: Chemicon^®
• technique(s): ELISA: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• NCBI accession no.: NM_003018.3
• UniProt accession no.: P11686
• shipped in: dry ice
• Gene Information: human ... SFTPC(6440) , SP5(389058)

Description

General description

Pulmonary surfactant is a complex mixture of phospholipids and proteins secreted from airway epithelial cells (type II cells) in alveoli which reduces the surface tension at the alveolar air liquid interface, providing alveolar stability necessary for normal ventilation. Four distinct proteins isolated from pulmonary surfactant are termed surfactant proteins A, B, C and D. SP A (28 - 36 kDa) and SP D (43 kDa) are collagenous carbohydrate binding proteins, whereas SP B (8 - 9 kDa) and SP C (4 kDa) are non collagenous hydrophobic proteins. SP C is a fragment of a much larger precursor protein of 21 kDa. The precursor contains an exceedingly hydrophobic region of 34 amino acids that comprises most of the mature SP C, and a unique poly-valine domain.

Specificity

Expected to cross-react with human.

Reacts strongly with proSP-C from human and mouse. Immunoprecipitates the 21 kDa proprotein as well as processing intermediates of M.W. 26 kDa, 21 kDa, 16 kDa and 14 kDa. Preincubation of the antiserum with the fusion protein results in ablation of immunostaining. On human tissue immunostains alveolar Type II cells in normal lung. The antibody also recognizes the aberrant form of proSP-C (6-12 kDa peptide) found in BAL and in alveolar proteinosis material on sections of lung samples from human patients with hereditary SP-B deficiency. On mouse tissue immunostains alveolar Type II cells in normal lung.

Immunogen

A synthetic peptide corresponding to amino acids 1-32 from the N-terminal of the human SP-C proprotein coupled to KLH

Application

Anti-Prosurfactant Protein C (proSP-C) Antibody detects level of Prosurfactant Protein C (proSP-C) and has been published & validated for use in ELISA, Immunohistochemistry (IHC), Immunohistochemistry - Paraffin (IHC-P) and Western Blotting (WB).

Immunohistochemistry:
1:2,000-1:4,000 dilution of a previous lot was used on human tissue (see protocol), 1:4,000 dilution on adult mouse lung and 1:1,000-1:2,000 dilution on fetal mouse lung (see protocol).
Immunohistochemistry: 1:2,000-1:4,000 on human tissue with and without antigen retrieval (see protocol) and 1:1,000-1:2,000 on adult and 1:500-1:1,000 on fetal mouse lung (see notes).

Immunoblot:
1:5,000 (best if not reduced)

Western Blot Analysis:
a previous lot used a dilution of 1:500

ELISA:
1:50,000

IMMUNOHISTOCHEMISTRY :
Notes:
1) Dilutions used are for normal (i.e. uninjured) adult mouse lung and have been used on FVB/N, Balb/c, nu/nu and SCID mice. The use of antigen retrieval systems to enhance immunostaining for this antibody is not necessary in the mouse lung and may actually increase background staining.

Optimal dilutions must be determined by the end user.

Research Category
Metabolism

Research Sub Category
Ion & Transport Channels

Quality

Routinely evaluated by Western Blot on rat lung lysates.

Western Blot Analysis: 1:500 dilution of this lot detected Prosurfactant Protein C on 10 μg of rat lung lysates

Target description

21 kDa proprotein as well as processing intermediates of M.W. 26 kDa, 21 kDa, 16 kDa and 14 kDa

Linkage

Replaces: 07-647

Physical form

Polyclonal rabbit serum containing 0.01% sodium azide as a preservative.

Unpurified

Storage and Stability

Stable at -20ºC in undiluted aliquots for up to twelve months after date of receipt. Avoid repeated freeze/thaw cycles.

Analysis Note

Control
Mouse lung epithelial cells.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable