AB3796

Anti-5′-Lipoxygenase Antibody

Chemicon^®, from rabbit

• Synonym(s): Arachidonate 5-lipoxygenase, 5-LO
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: rabbit, rat, mouse, human
• manufacturer/tradename: Chemicon^®
• technique(s): ELISA: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• NCBI accession no.: NM_000698.2
• UniProt accession no.: P09917
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... ALOX5(240)

Description

General description

5′-Lipoxygenase is a critical enzyme in the formation of leukotrienes from arachidonic acid. Upon activation, the enzyme translocates to either a nuclear or plasma membrane compartment.

Specificity

5′-Lipoxygenase

Immunogen

Purified human 5′-Lipoxygenase

Application

Anti-5′-Lipoxygenase Antibody detects level of 5′-Lipoxygenase & has been published & validated for use in ELISA, IP & WB.

Western blot: 1:1,000. Reacts with a 79 kDa band
Immunoprecipitation: 5 μL/mL lysate
ELISA: 1:1000 – 1:100,000
Optimal working dilutions must be determined by the end user.

Physical form

Format: Purified

Purified immunoglobulin in PBS with 50% Glycerol and 0.02% sodium azide

Analysis Note

Control
POSITIVE CONTROL: placenta, lung, and leukocytes.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 2