Choline acetyltranferase. The antibody will stain cholinergic neurons in the rat central and peripheral nervous systems.
Immunogen
A 22 amino acid synthetic peptide from porcine ChAT, coupled to KLH. The peptide sequence is GLFSSYRLPGHTQDTLVAQKSS. Due to sequence similarity, the antibody is expected to react with pig, mouse primate and human ChAT.
Application
Detect Choline Acetyltransferase using this Anti-Choline Acetyltransferase Antibody validated for use in ELISA, IH.
Immunohistochemistry: 1:2,000-1:4,000 in rat basal forebrain.
Optimal working dilutions must be determined by the end user.
Physical form
Affinity purified immunoglobulin. Lyophilized. Contains no preservative. Reconstitute with 20 μL of sterile distilled water. Centrifure to remove any insoluble material.
Storage and Stability
Maintain lyophilized material dry at -70°C for up to 12 months after date of receipt. After reconstitution, maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles. Glycerol (1:1) can be added for additional stability.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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