AB5042P

Anti-Choline Acetyltransferase Antibody

CHEMICON^®, rabbit polyclonal

• Synonym(s): ChAT
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• Clone: polyclonal
• Species reactivity: rat
• Application: ELISA, IHC
• Citations: 6

Properties

• Product Name: Anti-Choline Acetyltransferase Antibody, Chemicon^®, from rabbit
• biological source: rabbit
• Quality Level: 100
• antibody form: affinity purified immunoglobulin
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: rat
• manufacturer/tradename: Chemicon^®
• technique(s): ELISA: suitable, immunohistochemistry: suitable
• NCBI accession no.: NM_020549.3, NM_020984.2, NM_020985.2, NM_020986.2
• UniProt accession no.: P28329
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: rat ... Chat(290567)

Description

Immunogen

A 22 amino acid synthetic peptide from porcine ChAT, coupled to KLH. The peptide sequence is GLFSSYRLPGHTQDTLVAQKSS. Due to sequence similarity, the antibody is expected to react with pig, mouse primate and human ChAT.

Application

Detect Choline Acetyltransferase using this Anti-Choline Acetyltransferase Antibody validated for use in ELISA, IH.

Immunohistochemistry: 1:2,000-1:4,000 in rat basal forebrain.

Optimal working dilutions must be determined by the end user.

Biochem/physiol Actions

Choline acetyltranferase. The antibody will stain cholinergic neurons in the rat central and peripheral nervous systems.

Physical form

Affinity purified immunoglobulin. Lyophilized. Contains no preservative. Reconstitute with 20 μL of sterile distilled water. Centrifure to remove any insoluble material.

Preparation Note

Maintain lyophilized material dry at -70°C for up to 12 months after date of receipt. After reconstitution, maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles. Glycerol (1:1) can be added for additional stability.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable