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AB5320

Sigma-Aldrich

Anti-NG2 Chondroitin Sulfate Proteoglycan Antibody

Chemicon®, from rabbit

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Synonym(s):
Chondroitin sulfate proteoglycan NG2, Melanoma chondroitin sulfate proteoglycan 3, Melanoma-associated chondroitin sulfate proteoglycan, chondroitin sulfate proteoglycan 4, chondroitin sulfate proteoglycan 4 (melanoma-associated)
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

rat, mouse, monkey, human

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... CSPG4(1464)
mouse ... Cspg4(121021)
rat ... Cspg4(81651)
rhesus monkey ... Cspg4(713086)

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This Item
AB5320BAB5320C3N8912
antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified immunoglobulin

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

132.38, monoclonal

species reactivity

rat, mouse, monkey, human

species reactivity

rat

species reactivity

rat

species reactivity

rat

manufacturer/tradename

Chemicon®

manufacturer/tradename

-

manufacturer/tradename

-

manufacturer/tradename

-

technique(s)

ELISA: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

immunocytochemistry: suitable, immunohistochemistry: suitable

technique(s)

immunocytochemistry: suitable, immunohistochemistry: suitable

technique(s)

immunocytochemistry: suitable, immunoprecipitation (IP): suitable, indirect ELISA: suitable, microarray: suitable, western blot: 1 μg/mL using rat brain neonatal extract

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General description

Chondroitin sulfate proteoglycan 4 (UniProt: Q00657; also known as Chondroitin sulfate proteoglycan NG2, HSN tumor-specific antigen) is encoded by the Cspg4 (also known as Ng2) gene (Gene ID: 81651) in rat. Chondroitin sulfate proteoglycan NG2 is a single-pass type I membrane protein that plays a role in cell proliferation and migration and stimulates endothelial cells motility during microvascular morphogenesis. It is present in neural cells and in extraneural tissues, especially in the developing mesenchyme. Its level of expression is highest on immature, proliferating cells and decreases when these cells begin to differentiate. It is found on the surfaces of an unusual class of glial cells within the developing and mature central nervous system that have the properties of oligodendrocyte precursor cells. It is a major chondroitin sulfate proteoglycan that is produced after spinal cord injury and is expressed by macrophages and oligodendrocyte progenitors. It is also reported to inhibit neurite outgrowth and growth cone collapse during axon regeneration. NG2 chondroitin sulfate proteoglycan is synthesized with a signal peptide (aa 1-29), which is subsequently cleaved off in the mature form.(Ref.: Jones, LL et al. (2002). J. Neurosci. 22(7):2792-2803).

Specificity

NG2 Chondroitin Sulfate Proteoglycan. AB5320 identifies both the intact proteoglycan and the core protein by Western blot and ELISA. When oligodendrocyte precursor cells (i.e. O-2A progenitor cells) are stained alive, the stain appears as clusters on the cell surface. This antibody does not stain differentiated oligodendrocytes well.

Immunogen

Immunoaffinity purified NG2 Chondroitin Sulfate Proteoglycan from rat.

Application

Detect NG2 Chondroitin Sulfate Proteoglycan using this Anti-NG2 Chondroitin Sulfate Proteoglycan Antibody validated for use in ELISA, IC, IH, IP and WB.
Immunocytochemistry:
A 1:150-1:600 dilution of a previous lot was used in IC.

Immunohistochemistry:
1:200 dilution of a previous lot was used on frozen sections of embryonic mouse brain using an Alexa Fluor conjugated secondary antibody. Care should be taken to avoid overfixing tissue sections. A short (5-10 minute) fix in 2-4% PFA is recommended.

Immunoprecipitation:
2 μg/mL concentration of a previous lot was used in IP.

ELISA:
A 1:1,500-1:3,000 dilution of a previous lot was used in ELISA.

Optimal working dilutions and protocols must be determined by end user
Research Category
Neuroscience
Research Sub Category
Neuronal & Glial Markers

Quality

Routinely evaluated by Western Blot on rat brain lysates

Western Blot: 1:1000 dilution of this lot detected NG2 chondroitin sulfate on 10 μg of rat brain lysates.

Target description

270-300 kDa

Physical form

Antibody purified by protein A, then placed over a fibronectin affinity column.
Purified rabbit polyclonal in PBS buffer containing 0.02% azide.

Storage and Stability

Stable for 6 months at -20ºC in undiluted aliquots from date of receipt.

Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
Human melanoma, glioma and proliferating brain endothelial cells, rat B49 glial cell extracts or embryonic mouse brain tissue.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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SDF-1? induces PDGF-B expression and the differentiation of bone marrow cells into pericytes.
Hamdan, R; Zhou, Z; Kleinerman, ES
Molecular Cancer Research null
PDGF-C induces maturation of blood vessels in a model of glioblastoma and attenuates the response to anti-VEGF treatment.
di Tomaso, E; London, N; Fuja, D; Logie, J; Tyrrell, JA; Kamoun, W; Munn, LL; Jain, RK
Testing null
Aram F Hezel et al.
Cancer research, 72(18), 4840-4845 (2012-07-13)
The TGF-β pathway is under active consideration as a cancer drug target based on its capacity to promote cancer cell invasion and to create a protumorigenic microenvironment. However, the clinical application of TGF-β inhibitors remains uncertain as genetic studies show
Effects of an anti-VEGF-A monoclonal antibody on laser-induced choroidal neovascularization in mice: optimizing methods to quantify vascular changes.
Campa, C; Kasman, I; Ye, W; Lee, WP; Fuh, G; Ferrara, N
Investigative Ophthalmology & Visual Science null
Ginger Tsueng et al.
Journal of virology, 85(12), 5718-5732 (2011-04-08)
Enteroviruses, including coxsackieviruses, exhibit significant tropism for the central nervous system, and these viruses are commonly associated with viral meningitis and encephalitis. Previously, we described the ability of coxsackievirus B3 (CVB3) to infect proliferating neuronal progenitor cells located in the

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Protocols

Tips and troubleshooting for FFPE and frozen tissue immunohistochemistry (IHC) protocols using both brightfield analysis of chromogenic detection and fluorescent microscopy.

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