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AB5467

Sigma-Aldrich

Anti-AANAT Antibody

serum, Chemicon®

Synonym(s):

Arylalkylamine N-acetyltransferase, SNAT, Serotonin Acetylase, EC 2.3.1.87

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

rat, sheep, fish, human, bovine

manufacturer/tradename

Chemicon®

technique(s)

western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... AANAT(15)

Specificity

Recognizes AANAT (Arylalkylamine N-acetyltransferase). The antibody specifically recognizes the phosphorylated N-terminal PKA site of AANAT (pT31) (Ganguly et al. 2005). The antibody reacts with natural and recombinant protein.

Immunogen

Synthetic peptide (phosphorylated) corresponding to amino acids near the N-terminous of rat AANAT conjugated to BSA. {Ganguly, S et al, 2005}.

Application

Anti-AANAT Antibody is an antibody against AANAT for use in WB.
Research Category
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
Western Blot: 1:10,000-1:20,000 using ECL. Reacts with the ~23 kDa AANAT protein. Some reactivity may also be seen at ~97 kDa. Suggested blocking buffer is PBS with 10% non fat dry milk and 0.2% Tween-20. Suggested dilution buffer is PBS with 1 mg/mL BSA fraction V. Suggested incubation time is overnight at room temperature (~23°C).

Optimal working dilutions must be determined by the end user.

Physical form

Liquid rabbit serum containing no preservative.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

Analysis Note

Control
POSITIVE CONTROL:

Night adapted rat pineal gland. Expression is light sensitive; highest levels are obtained from animals at night.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

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Jinglin Zhang et al.
Journal of pineal research, 65(1), e12481-e12481 (2018-02-27)
Melatonin is a key hormone that regulates circadian rhythms, metabolism, and reproduction. However, the mechanisms of melatonin synthesis and secretion have not been fully defined. The purpose of this study was to investigate the functions of the LIM homeobox transcription
Ahmed Soliman et al.
Journal of pineal research, 59(1), 38-46 (2015-04-03)
Melatonin is highly produced in the placenta where it protects against molecular damage and cellular dysfunction arising from hypoxia/re-oxygenation-induced oxidative stress as observed in primary cultures of syncytiotrophoblast. However, little is known about melatonin and its receptors in the human

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