AB5475

Anti-Prox 1 Antibody

serum, Chemicon^®

• Synonym(s): Anti-A230003G05Rik, Anti-PROX-1
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: serum
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: rat, mouse, zebrafish
• species reactivity (predicted by homology): human
• manufacturer/tradename: Chemicon^®
• technique(s): immunocytochemistry: suitable; immunohistochemistry: suitable
• NCBI accession no.: NM_002763.3
• UniProt accession no.: Q92786
• shipped in: dry ice
• Gene Information: human ... PROX1(5629)

Description

General description

Prox1, the homolog of Drosophila Prospero, is a homeobox-containing transcription factor that binds and functions as a co-receptor of liver receptor homolog 1 (LRH1/NR5A2). It is a specific marker for lymphatic endothelial cells, as well as hepatoblasts and hepatocytes. Human Prox1 shares 97% amino acid sequence identity with mouse and rat Prox1.

Specificity

Other species have not been tested.

Recognizes Prox1. The antibody is useful for identifying Prox1 expressing cells during the development of many areas of the nervous system including the dorsal spinal cord, ganglionic eminences, thalamus and hippocampus.

Immunogen

Synthetic peptide from the C-terminus of mouse Prox1.

Application

Detect Prox 1 using this Anti-Prox 1 Antibody validated for use in IC, IH.

Immunohistochemistry:
1:2,000-1:10,000 with overnight incubation on 4% paraformaldehyde fixed, frozen tissue sections. For embryos immersion fixation is recommended. For older animals perfusion fixation is recommended. Suggested permeabilization solution is 0.1% Triton X100. Suggested blocking buffer is PBS with 0.1% Triton and 10% serum.

Immunocytochemistry:
A previous lot of this antibody was used on Immunocytochemistry

Physical form

Rabbit serum in 50 % glycerol.

Storage and Stability

Stable for 1 year at -20°C from date of receipt. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Analysis Note

Control
Embryonic brain tissue.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1