AB5543

Anti-MAP2 Antibody

CHEMICON^®, chicken polyclonal

• Synonym(s): Anti-MAP-2, Anti-MAP2A, Anti-MAP2B, Anti-MAP2C
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• Clone: polyclonal
• Species reactivity: rat
• Application: ICC, IHC, WB
• Citations: 84

Properties

• Product Name: Anti-MAP2 Antibody, Chemicon^®, from chicken
• biological source: chicken
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: rat
• manufacturer/tradename: Chemicon^®
• technique(s): immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable
• NCBI accession no.: NM_001039538.1, NM_002374.3, NM_031845.2, NM_031847.2
• UniProt accession no.: P11137
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: rat ... Map2(25595)

Description

General description

Recognizes a doublet of ~280 kDa and a doublet of ~70kDa

Immunogen

Purified bovine brain MAP2

Application

Research Category
Neuroscience

Anti-MAP2 Antibody is an antibody against MAP2 for use in IC, IH & WB.

Research Sub Category
Neuronal & Glial Markers

Neurofilament & Neuron Metabolism

Western blot: 1:5,000-1:10,000 using ECL. Recognizes a doublet of ~280 kDa and a doublet of ~70kDa.

Immunohistochemistry on frozen tissue sections. Suggested fixative is 4% paraformaldehyde (1-10 minutes) followed by 5 minutes in -20°C methanol.

Immunocytochemistry: 1:5,000-1:10,000 on cells in tissue culture fixed for 1-10 minutes in 10% formalin followed by five minutes in -20°C methanol.

Optimal working dilutions must be determined by the end user.

Biochem/physiol Actions

Microtubule Associated Protein 2 (MAP2). It is expected that the antibody will react with all mammals.

Physical form

Ammonium Sulfate Precipitation

Format: Purified

Purified chicken IgY. Liquid in PBS containing 5 mM sodium azide.

Preparation Note

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Positive = cerebral cortex. Negative = liver or kidney.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 2