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AB5603

Sigma-Aldrich

Anti-Sox2 Antibody

Chemicon®, from rabbit

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Synonym(s):
Transcription factor SOX-2, Sex determining region Y-box 2, SRY-related HMG-box gene 2, Transcription factor SOX2
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100
300

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human, mouse

species reactivity (predicted by homology)

rhesus macaque (based on 100% sequence homology), rat (based on 100% sequence homology), bovine (based on 100% sequence homology)

packaging

antibody small pack of 25 μg

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

ambient

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... SOX2(6657)

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This Item
SAB5700644S9072S1451
Anti-Sox2 Antibody Chemicon®, from rabbit

AB5603

Anti-Sox2 Antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

purified from hybridoma cell culture

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

SOX2-6, monoclonal

species reactivity

human, mouse

species reactivity

rat, human, mouse

species reactivity

human, rat, mouse

species reactivity

human, mouse

Gene Information

human ... SOX2(6657)

Gene Information

human ... SOX2(6657)

Gene Information

human ... SOX2(6657)
mouse ... Sox2(20674)
rat ... Sox2(499593)

Gene Information

human ... SOX2(6657)
mouse ... Sox2(20674)
rat ... Sox2(499593)

UniProt accession no.

P48431

UniProt accession no.

P48431

UniProt accession no.

P48431

UniProt accession no.

P48431

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General description

Transcription factor SOX-2 (UniProt: P48431; also known as SOX-2, Sex determining region Y-box 2, SRY-related HMG-box gene 2) is encoded by the SOX2 gene (Gene ID: 6657) in human. SOX-2 is a transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. It is shown to be critical for early embryogenesis and for embryonic stem cell pluripotency. It may function as a switch in neuronal development. It allows neural cells in undifferentiated state by counteracting the activity of proneural proteins and suppresses neuronal differentiation. Sumoylation of SOX-2 is shown to inhibit binding on DNA and negatively regulate the FGF4 transactivation. Defects in SOX2 gene are linked to microphthalmia that is characterized by eye malformation ranging from small eye size of a single eye to complete bilateral absence of ocular tissue.

Specificity

Anti-Sox2 Antibody recognizes Sox2 C-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to a C-terminal region sequence of human Sox2.

Application

Anti-SOX2 Antibody, Cat. No. AB5603, is a highly specific rabbit polyclonal antibody SOX2 and has been tested for use in Immunocytochemistry, and Immunohistochemistry (Paraffin), and Western Blotting.
Immunocytochemistry Analysis: 10 µg/mL from a representative lot detected Sox2 in H9 human stem cells.
Immunocytochemistry Analysis: A representative lot detected nuclear Sox2 immunoreactivity in 4% paraformaldehyde-fixed, 0.1% Triton X-100-permeabilized human embryonic stem cells (hESCs) by fluorescent immunocytochemistry (Kingham, E., et al. (2013). Small. 9(12):2140-2151).
Immunocytochemistry Analysis: A representative lot detected Sox2 immunoreactivity by fluorescent immunocytochemistry staining of 4% paraformaldehyde-fixed iPSCs generated from human kidney tubular renal epithelial cells (Montserrat, N., et al. (2012). J. Biol. Chem. 287(29):24131-24138).
Immunocytochemistry Analysis: A representative lot detected Sox2 immunoreactivity by fluorescent immunocytochemistry staining of 4% paraformaldehyde-fixed, 0.5% Triton X-100-permeabilized iPSCs generated from the skin cells of patients with Parkinson′s disease (Sánchez-Danés, A., et al. (2012). EMBO Mol. Med. 4(5):380-395).
Immunocytochemistry Analysis: Representative lots detected Sox2 immunoreactivity by fluorescent immunocytochemistry staining of 4% paraformaldehyde-fixed, 0.2% Triton X-100-permeabilized iPSCs generated from mouse ear cells and MEF (Burns, J.C., et al. (2012). PLoS One. 7(10):e48704; Hadadeh, O., et al. (2012). PLoS One. 7(11):e49065).
Western Blotting Analysis: A representative lot detected Sox2 in mouse embryonic stem cell (mESC) nuclear extracts (Banaszynski, L.A., et al. (2013). Cell. 155(1):107-120).
Immunofluorescence Analysis: A representative lot detected Sox2-positive cells in 4% paraformaldehyde-fixed, 0.3% Triton X-100-permeabilized mouse brain vibratome sections by fluorescent immunohistochemistry (Matsuda, S., et al. (2012). J. Neurosci. 32(36):12543-12557).
Immunohistochemistry Analysis: A representative lot detected Sox2 immunoreactivity in 4% paraformaldehyde-fixed whole mount mouse embryo sections (van Rooijen, C., et al. Development. 139(14):2576-2583).

Quality

Evaluated by Western Blotting in mouse embryonic stem cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected SOX2 in 10 µg of mouse embryonic stem cell lysate.

Target description

~39 kDa observed. 34.31 kDa (human) and 34.45 (mouse) calculated. Uncharacterized band(s) may appear in some lysates

Linkage

Replaces: AB5770

Analysis Note

Control
Immunoblot: Mouse or human embryonic stem cell lysate, mouse embryonic germ cell lysate
Immunocytochemistry: Human embryonic stem cells (H9 line)

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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The Sox2 high mobility group transcription factor inhibits mature osteoblast function in transgenic mice.
Holmes, G; Bromage, TG; Basilico, C
Bone null
Stage-dependent modes of Pax6-Sox2 epistasis regulate lens development and eye morphogenesis.
Smith, AN; Miller, LA; Radice, G; Ashery-Padan, R; Lang, RA
Development null
A novel embryonic stem cell line derived from the common marmoset monkey (Callithrix jacchus) exhibiting germ cell-like characteristics.
Muller, T; Fleischmann, G; Eildermann, K; Matz-Rensing, K; Horn, PA; Sasaki, E; Behr, R
Human Reproduction null
Astrocytes derived from fetal neural progenitor cells as a novel source for therapeutic adenosine delivery.
Van Dycke, A; Raedt, R; Verstraete, A; Theofilas, P; Wadman, W; Vonck, K; Boison, D; Boon, P
Seizure : the Journal of the British Epilepsy Association null
Jianwen Que et al.
Development (Cambridge, England), 136(11), 1899-1907 (2009-05-01)
The esophagus, trachea and lung develop from the embryonic foregut, yet acquire and maintain distinct tissue phenotypes. Previously, we demonstrated that the transcription factor Sox2 is necessary for foregut morphogenesis and esophagus development. We show that Sox2 is also required

Articles

ReNcell neural progenitors are immortalized human neural stem cell lines that can differentiate into neurons, astrocytes sand oligodendrocytes.

Human iPSC neural differentiation media and protocols used to generate neural stem cells, neurons and glial cell types.

Protocols

Step-by-step culture protocols for neural stem cell culture including NSC isolation, expansion, differentiation and characterization.

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