AB5935

Anti-Tyrosine Hydroxylase Antibody, phosphoSer 40

Chemicon^®, from rabbit

• Synonym(s): Tyrosine Monooxygenase
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Clone: polyclonal
• Species reactivity: avian, rat, mouse
• Application: ICC, IHC, WB
• Citations: 36

Properties

• biological source: rabbit
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: avian, rat, mouse
• species reactivity (predicted by homology): quail (100% sequence homology), nonhuman primates (100% sequence homology), human (100% sequence homology), porcine (100% sequence homology)
• manufacturer/tradename: Chemicon^®
• technique(s): immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable
• NCBI accession no.: NM_000360.3
• UniProt accession no.: P07101
• shipped in: dry ice
• target post-translational modification: phosphorylation (pSer40)
• Quality Level: 100
• Gene Information: human ... TH(7054) ; rat ... Th(25085)

Description

General description

60 kDa

Tyrosine hydroxylase plays an important role in the physiology of adrenergic neurons. It is the first and rate-limiting enzyme involved in the biosynthesis of the catecholamines Dopamine and Norepinephrine from tyrosine. TH is, therefore, a useful marker for dopaminergic and noradrenergic neurons. The enzymatic activity of TH requires ferrous ions as cofactors and is believed to be regulated by phosphorylation. At least four isoforms of human TH have been identified which result from alternative splicing.

Immunogen

Epitope: phospho-Ser40

Synthetic peptide from the phosphoSer40 of rat Tyrosine Hydroxylase.

Application

Anti-Tyrosine Hydroxylase Antibody, phosphoSer 40 detects level of Tyrosine Hydroxylase & has been published & validated for use in IC, IH & WB.

Immunohistochemistry:
A 1:1,000 dilution of a previous lot was used in IH.

Immunocytochemistry:
A 1:1,000 dilution of a previous lot was used in IC.

Optimal working dilutions must be determined by the end user.

Research Category
Neuroscience

Research Sub Category
Neurotransmitters & Receptors

Biochem/physiol Actions

Other species have not been tested.

Tyrosine Hydroxylase, phosphoSer40. The antibody recognizes a protein of 60 kDa corresponding to TH phosphorylated at Ser40. Depending on brain region being studied, protein loads and detection methods used some higher molecular weight bands may be detected.

Physical form

ImmunoAffinity Purified

Purified rabbit polyclonal liquid in buffer containing 10mM Hepes, pH 7.5, 150mM NaCl, 100 µg/mL BSA, and 50% glycerol.

Preparation Note

Stable for 6 months at -20°C in undiluted from date of receipt.

Analysis Note

Control
POSITIVE CONTROL: brain (sympathetic nerve terminals) and adrenal glands.

Routinely evaluated by Western Blot on PC12 lysates.

Western Blot Analysis:
1:500 dilution of this lot detected TYROSINE HYDROX PSER40 on 10 μg of PC12 lysates.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 2