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AB6046

Sigma-Aldrich

Anti-Cytoplasmic FMR1-interacting protein 1 Antibody

from rabbit, purified by affinity chromatography

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Synonym(s):
cytoplasmic FMR1 interacting protein 1, Specifically Rac1-associated protein 1, cytoplasmic FMRP interacting protein 1, cytoplasmic FMR1-interacting protein 1, selective hybridizing clone
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse, human

species reactivity (predicted by homology)

bovine (based on 100% sequence homology), rat (based on 100% sequence homology), primate (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology)

technique(s)

immunocytochemistry: suitable
western blot: suitable

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... SRA1(10011)

General description

Cytoplasmic FMR1-interacting protein 1 (CYFIP1) is a member of the CYFIP family. It is a component of the CYFIP1-EIF4E-FMR1 complex, a regulatory complex for translational repression that binds to the mRNA cap. Within the complex, CYFIP1 serves as an adapter between the other two components and upregulates the translation repression activity of FMR1. It regulates epithelial morphogenesis, binds F-actin, and is a component in the development and formation of axon outgrowth, lamellipodia, protrusions, and membrane ruffles. Through its interaction with RAC1, CYFIP1 regulates the activity of WASP family members. CYFIP1 is associated with Prader-Willi/Angelman syndromes and may also have a role in autism.

Immunogen

Epitope: Unknown
KLH-conjugated linear peptide corresponding to an internal sequence (a.a.519-532) of human Cytoplasmic FMR1-interacting protein 1.

Application

Detect Cytoplasmic FMR1-interacting protein 1 using this Anti-Cytoplasmic FMR1-interacting protein 1 Antibody validated for use in WB & IC.
Research Category
Cell Structure

Signaling
Research Sub Category
Cytoskeletal Signaling
Western Blot (Snap i.d.) Analysis: 5 µg/mL from a previous lot detected Cytoplasmic FMR1-interacting protein 1 in 10 µg of HeLa cell lysate.
Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected Cytoplasmic FMR1-interacting protein 1 in NIH/3T3, HeLa, and A431 cells.

Quality

Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 1 µg/mL of the antibody detected Cytoplasmic FMR1-interacting protein 1 on 10 µg of HeLa cell lysate.

Target description

~ 154 kDa observed. There are three noted isoforms with calculated MWs of 51 kDa, 94 kDa, and 145 kDa Additional isoforms may be observed in some lysates.

Physical form

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Analysis Note

Control
HeLa cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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