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AB761

Sigma-Aldrich

Anti-Collagen Type II Antibody

Chemicon®, from rabbit

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Synonym(s):
Anti-Anti-ANFH, Anti-Anti-AOM, Anti-Anti-COL11A3, Anti-Anti-SEDC, Anti-Anti-STL1
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

NCBI accession no.

NM_001844.4
NM_033150.2

UniProt accession no.

P02458

shipped in

wet ice

Gene Information

human ... COL2A1(1280)

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AB745SAB1403684MAB3303
Sigma-Aldrich

Sigma-Aldrich

AB761

Anti-Collagen Type II Antibody

Sigma-Aldrich

Sigma-Aldrich

AB745

Anti-Collagen Type I Antibody

Monoclonal Anti-COL2A1 antibody produced in mouse clone 1E1, purified immunoglobulin, buffered aqueous solution

Sigma-Aldrich

SAB1403684

Monoclonal Anti-COL2A1 antibody produced in mouse

Anti-Collagen Type VI Antibody, clone VI-26 clone VI-26, Chemicon®, from mouse

Sigma-Aldrich

MAB3303

Anti-Collagen Type VI Antibody, clone VI-26

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin (Semi-purified)

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

clone

polyclonal

clone

polyclonal

clone

1E1, monoclonal

clone

VI-26, monoclonal

species reactivity

human

species reactivity

human

species reactivity

human

species reactivity

human, rabbit

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

manufacturer/tradename

-

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable

technique(s)

ELISA: suitable, immunohistochemistry: suitable, western blot: suitable

technique(s)

indirect ELISA: suitable, western blot: 1-5 μg/mL

technique(s)

ELISA: suitable, immunohistochemistry: suitable (paraffin)

Specificity

The antibody reacts with both native and denatured human collagen type II.



Cross Reactivity Percent

Human collagen type II 90%

Human collagen type I, III, IV, V <1%

Human plasma proteins do not interfere with binding to collagen.

Immunogen

Collagen type II extracted and purified from human cartilage.

Application

Anti-Collagen Type II Antibody detects level of Collagen Type II & has been published & validated for use in ELISA, WB, IC, IH.
ELISA 1:1000.

Indirect immunofluorescence on cryostat sections or cultured cells 1:10-1:20

Western Blot 1:100

Optimal working dilutions must be determined by the end user.
Research Category
Cell Structure
Research Sub Category
ECM Proteins

Physical form

Rabbit antiserum passed over agarose sorbents with immobilized human blood plasma proteins and human collagen types I, III, IV and V. Antibody was affinity purified on immobilized human collagen type II.

Product is liquid in 0.15M sodium chloride, 10mM sodium phosphate pH 7.5 with 0.1% mannitol and 0.1% dextran as stabilizers. No preservative.

Storage and Stability

Maintain at 2-8°C for up to 12 months from date of reciept. Remove any nondispersed aggregates by microcentrifugation prior to use.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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