MilliporeSigma
All Photos(3)

AB765P

Sigma-Aldrich

Anti-Mouse Collagen Type I Antibody

Chemicon®, from rabbit

Synonym(s):
Anti-OI3, Anti-EDSC, Anti-OI4, Anti-OI1, Anti-OI2, Anti-CAFYD
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

mouse

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunohistochemistry: suitable
radioimmunoassay: suitable
western blot: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

dry ice

Gene Information

human ... COL1A1(1277)

General description

Collagen is the main protein of connective tissue in animals and the most abundant protein in mammals, making up about 25% of the total protein content. It is one of the long, fibrous structural proteins whose functions are quite different from those of globular proteins such as enzymes; tough bundles of collagen called collagen fibers are a major component of the extracellular matrix that supports most tissues and gives cells structure from the outside, but collagen is also found inside certain cells. Collagen has great tensile strength, and is the main component of fascia, cartilage, ligaments, tendons, bone and teeth. Along with soft keratin, it is responsible for skin strength and elasticity, and its degradation leads to wrinkles that accompany aging. It strengthens blood vessels and plays a role in tissue development. It is present in the cornea and lens of the eye in crystalline form. Collagen occurs in many places throughout the body. There are 12 types of collagen described in literature. Collagen Type 1: This is the most abundant collagen of the human body. It is present in scar tissue, the end product when tissue heals by repair. It is found in tendons, the endomysium of myofibrils and the organic part of bone. Collagen Type 4: It is present in basal lamina and the eye lens. Also serves as part of the filtration system in capillaries and the glomeruli of nephron in the kidney.
Collagen Type I extracted and purified from mouse skin. Antibody shows less than 0.1% reactivity with mouse Collagen Types II, IV and with human, rat and chicken Collagens Type I in addition to a 1.0% reactivity with mouse Collagen Type III.

Specificity

Antibody shows less than 0.1% reactivity with mouse Collagen Types II, IV and with human, rat and chicken Collagens Type I in addition to a 1.0% reactivity with mouse Collagen Type III.
Recognizes mouse Collagen Type I.

Immunogen

Collagen Type I extracted and purified from mouse skin.

Application

Research Category
Cell Structure
Anti-Mouse Collagen Type I Antibody detects level of Mouse Collagen Type I and has been published in over 20 citations and validated for use in ELISA, IH, RIA and WB.
Western Blot Analysis:
1:500 dilution of a previous lot detected Collagen Type I on 10 μg of Mouse Liver lysate.

Immunohistochemistry:
1:40 dilution of a previous lot for immunofluorescent staining of frozen mouse skin and liver tissues.

RIA:
A 1:200 dilution of a previous lot was used in RIA.

ELISA:
A previous lot of this antibody was used in ELISA
Research Sub Category
ECM Proteins

Target description

MW of Collagen Type I precursor: 140-210 kDa MW of mature Collagen Type I: 70-90 kDa

Linkage

Replaces: AB765

Physical form

Purified rabbit polyclonal serum in buffer containing 0.01 M phosphate, 0.09 M NaCl, pH 7.2. No preservatives.
Format: Purified
Protein A purified

Storage and Stability

Stable for 1 year at -20ºC from date of receipt.

Analysis Note

Control
Mouse skin and mouse liver tissues

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Quality

Enter Lot Number to search for Certificate of Quality (COQ).

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