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AB769

Sigma-Aldrich

Anti-Collagen Type IV Antibody

Chemicon®, from goat

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Synonym(s):
Anti-Anti-BSVD, Anti-Anti-BSVD1, Anti-Anti-COL4A1s, Anti-Anti-PADMAL, Anti-Anti-RATOR
eCl@ss:
32160702
NACRES:
NA.41

biological source

goat

Quality Level

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human, bovine, mouse

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
dot blot: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable

suitability

not suitable for Western blot
not suitable for immunohistochemistry (Paraffin)

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... COL4A1(1282)

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This Item
MAB3326MAB2500AB758B
Anti-Collagen Type IV Antibody Chemicon®, from goat

AB769

Anti-Collagen Type IV Antibody

biological source

goat

biological source

mouse

biological source

mouse

biological source

goat

antibody form

affinity purified immunoglobulin

antibody form

purified antibody

antibody form

purified immunoglobulin

antibody form

affinity purified immunoglobulin

Gene Information

human ... COL4A1(1282)

Gene Information

human ... COL4A1(1282)

Gene Information

human ... COL7A1(1294)

Gene Information

human ... COL1A1(1277)

clone

polyclonal

clone

IV-4H12, monoclonal

clone

32, monoclonal, mAb-VII, monoclonal

clone

polyclonal

shipped in

wet ice

shipped in

dry ice

shipped in

wet ice

shipped in

wet ice

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Specificity

Recognizes Human and bovine type IV collagen as demonstrated by ELISA. Less than 10% cross reactivity with collagen types I, II, III, V, and VI. May show reactivity to type IV collagen from other species. AB769 has not been tested with other extracellular matrix proteins (e.g., laminin, fibronectin).

Immunogen

Human and bovine placental collagen type IV

Application

Detect Collagen Type IV using this Anti-Collagen Type IV Antibody validated for use in DB, ELISA, IC, IH.
Dot and slot blotting: 1:100-1:500

ELISA: 1:1000-1:4000

Indirect immunohistochemistry (frozen sections only): 1:10-1:40

Immunocytochemistry 1:10-1:20

Optimal working dilutions must be determined by the end user.
Research Category
Cell Structure
Research Sub Category
ECM Proteins

Physical form

Affinity Purified immunoglobulin. Prior to purification the antisera was adsorbed against collagen type I, II, III, V and VI immobilized on Sepharose 4B. Liquid in borate buffered saline, pH 8.2, no sodium azide.
ImmunoAffinity Purified

Storage and Stability

Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Positive Control: Kidney, muscle, tendon spleen tissue
Negative Control: Neurons/glia

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Sepharose is a trademark of Cytiva

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Repr. 1B

Storage Class

6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


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Localization of AQP5 during development of the mouse submandibular salivary gland.
Larsen, HS; Aure, MH; Peters, SB; Larsen, M; Messelt, EB; Kanli Galtung, H
Journal of Molecular Histology null
Christian G M van Dijk et al.
Circulation. Heart failure, 9(4), e002760-e002760 (2016-04-09)
The combination of cardiac and renal disease driven by metabolic risk factors, referred to as cardiorenal metabolic syndrome (CRMS), is increasingly recognized as a critical pathological entity. The contribution of (micro)vascular injury to CRMS is considered to be substantial. However
Abigail L Mackey et al.
Skeletal muscle, 7(1), 24-24 (2017-11-09)
While muscle regeneration has been extensively studied in animal and cell culture models, in vivo myogenesis in adult human skeletal muscle has not been investigated in detail. Using forced lengthening contractions induced by electrical stimulation, we induced myofibre injury in
Daniela Semeniak et al.
Journal of cell science, 129(18), 3473-3484 (2016-08-10)
Collagen receptors GPVI (also known as GP6) and integrin α2β1 are highly expressed on blood platelets and megakaryocytes, their immediate precursors. After vessel injury, subendothelial collagen becomes exposed and induces platelet activation to prevent blood loss. Collagen types I and
Francisco Caiado et al.
PloS one, 3(11), e3752-e3752 (2008-11-19)
Bone marrow (BM) derived vascular precursor cells (BM-PC, endothelial progenitors) are involved in normal and malignant angiogenesis, in ischemia and in wound healing. However, the mechanisms by which BM-PC stimulate the pre-existing endothelial cells at sites of vascular remodelling/recovery, and

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