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AB911

Sigma-Aldrich

Anti-Oxytocin Antibody

serum, Chemicon®

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Synonym(s):
Anti-OT, Anti-OT-NPI, Anti-OXT-NPI
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human, mouse, rat, rabbit, sheep, hamster

manufacturer/tradename

Chemicon®

technique(s)

dot blot: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
radioimmunoassay: suitable

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... OXTR(5021)

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This Item
MAB5296ABN297HPA071892
Anti-Oxytocin Antibody serum, Chemicon®

Sigma-Aldrich

AB911

Anti-Oxytocin Antibody

antibody form

serum

antibody form

ascites fluid

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

clone

polyclonal

clone

4G11, monoclonal

clone

polyclonal

clone

polyclonal

species reactivity

human, mouse, rat, rabbit, sheep, hamster

species reactivity

rat, human

species reactivity

mouse, human

species reactivity

human

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

manufacturer/tradename

-

manufacturer/tradename

-

technique(s)

dot blot: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable (paraffin), radioimmunoassay: suitable

technique(s)

ELISA: suitable, immunohistochemistry: suitable

technique(s)

western blot: suitable

technique(s)

immunohistochemistry: 1:500-1:1000

Specificity

Recognizes Oxytocin. Less than 1% cross-reactivity with arginine vasopressin.

Immunogen

Synthetic oxytocin (Sigma) conjugated to thyroglobulin.

Application

Anti-Oxytocin Antibody is an antibody against Oxytocin for use in DB, IC, IH(P), RIA.
Immunohistochemistry: 1:5,000-1:10,000 (18-24 hour incubation) on formalin, Bouin′s or Zamboni′s fixed tissue. Will react on paraffin embedded tissue.

Immunocytochemistry: 1:10,000 on fetal rat neurons.

Dot Blot: 1:1000

RIA

Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Research Sub Category
Hormones & Receptors

CNS Control of Metabolism

Physical form

Neat rabbit antisera. Liquid. Contains no preservative.
Unpurified

Storage and Stability

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Pituitary Gland

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Karin Warfvinge et al.
The journal of headache and pain, 21(1), 10-10 (2020-02-08)
Recent work, both clinical and experimental, suggests that the hypothalamic hormone oxytocin (OT) and its receptor (OTR) may be involved in migraine pathophysiology. In order to better understand possible central actions of OT in migraine/headache pathogenesis, we mapped the distribution
Mehwish Anwer et al.
Journal of neurotrauma, 37(7), 924-938 (2019-10-28)
Traumatic brain injury (TBI) causes damage to the hypothalamo-hypophyseal axis, leading to endocrine dysregulation in up to 40% of TBI patients. Hence, there is an urgent need to identify non-invasive biomarkers for TBI-associated hypothalamo-hypophyseal pathology. Sushi repeat-containing protein X-linked 2
Nicole Denoix et al.
Frontiers in neurology, 11, 649-649 (2020-08-06)
The hydrogen sulfide (H2S) and the oxytocin/oxytocin receptor (OT/OTR) systems interact in trauma and are implicated in vascular protection and regulation of fluid homeostasis. Acute brain injury is associated with pressure-induced edema formation, blood brain barrier disruption, and neuro-inflammation. The
Yu-Ting Lin et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 38(5), 1218-1231 (2017-12-28)
Oxytocin (OXT) receptors (OXTRs) are prominently expressed in hippocampal CA2 and CA3 pyramidal neurons, but little is known about its physiological function. As the functional necessity of hippocampal CA2 for social memory processing, we tested whether CA2 OXTRs may contribute
Mahasweta Das et al.
The Journal of comparative neurology, 500(4), 761-776 (2006-12-13)
The hypothalamic paraventricular nucleus (PVN) coordinates major neuroendocrine and behavioral mechanisms, particularly responses to homeostatic challenges. Parvocellular and magnocellular PVN neurons are richly innervated by pituitary adenylate cyclase-activating polypeptide (PACAP) axons. Our recent functional observations have also suggested that PACAP

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