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AB9748-I

Sigma-Aldrich

Anti-14-3-3 Antibody (pan)

from rabbit, purified by affinity chromatography

Synonym(s):
14-3-3 protein beta/alpha, KCIP-1, Protein 1054, Protein kinase C inhibitor protein 1, 14-3-3 protein epsilon, 14-3-3E, 14-3-3 protein eta, Protein AS1, 14-3-3 protein gamma, 14-3-3 protein theta, 14-3-3 protein T-cell, 14-3-3 protein tau, Protein HS1, 1
eCl@ss:
32160702

Quality Level

biological source

rabbit

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

species reactivity (predicted by homology)

chicken (based on 100% sequence homology), Xenopus (based on 100% sequence homology), fish (based on 100% sequence homology), bovine (based on 100% sequence homology), rat (based on 100% sequence homology), sheep (based on 100% sequence homology), zebrafish (based on 100% sequence homology), rabbit (based on 100% sequence homology), mouse (based on 100% sequence homology), porcine (based on 100% sequence homology)

technique(s)

immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable

shipped in

ambient

General description

14-3-3 protein beta/alpha (KCIP-1, Protein 1054, Protein kinase C inhibitor protein 1), epsilon (14-3-3E), eta (Protein AS1), gamma (KCIP-1, Protein kinase C inhibitor protein 1), theta (14-3-3 protein T-cell, 14-3-3 protein tau, Protein HS1), zeta/delta (KCIP-1, Protein kinase C inhibitor protein 1) are encoded by the YWHAB, YWHAE, YWHAH, YWHAG, YWHAQ, YWHAZ gene (Gene ID 7529, 7531, 7533, 7532, 10971, 7534, respectively) in human. Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers, thus rendering 14-3-3 as a key multifunctional regulatory molecule. 14-3-3 isomers have been implicated in several neurological disorders: for example isoforms gamma, epsilon and beta are expressed in the cerebrospinal fluid of Creutzfeldt-Jakob patients, but not patients suffering from other neurological conditions such as Alzheimer′s. The 14-3-3 pan antibody is one of a panel of antibodies that were generated against 14-3-3 isoforms and have demonstrated their potential for use as an ELISA-based diagnostic tool. These antibodies will allow the research to investigate the role of this protein in key cellular processes and neurological disorders such as Creutzfeldt-Jakob, Alzheimer′s and Parkinson′s disease.

Specificity

This antibody targets the N-terminal end sequences of 14-3-3 alpha/beta (YWHAB), gamma (YWHAG), zeta/delta (YWHAZ), epsilon (YWHAE), eta (YWHAH or YWHA1), and theta (YWHAQ).

Immunogen

A mixture of KLH-conjugated linear peptides corresponding the the N-terminal end sequences of human 14-3-3 alpha/beta (YWHAB), gamma (YWHAG), zeta/delta (YWHAZ), epsilon (YWHAE), eta (YWHAH or YWHA1), and theta (YWHAQ).

Application

Anti-14-3-3 pan, Cat. No. AB9748-I, is a highly specific rabbit polyclonal antibody, that targets 14-3-3 protein and has been tested in Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting and .
Immunohistochemistry Analysis: A 1:5,000 dilution from a representative lot detected 14-3-3 immunoreactivity in human prostate cancer, cerebellum, and tonsil tissue sections.

Immunoprecipitation Analysis: 5 µg from a representative lot immunoprecipitated 14-3-3 from 500 µg of A431 cell lysate.
Research Category
Neuroscience

Quality

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected 14-3-3 in 10 µg of A431 cell lysate.

Target description

~28 kDa observed. 28.08/27.85 kDa (human isoform Long/Short; YWHAB), 27.75 kDa (human YWHAZ), 29.17 kDa (human YWHAE), 28.22 kDa (human YWHAH or YWHA1), 28.30 kDa (human YWHAG), 27.76 kDa (human YWHAQ) calculated. Uncharacterized bands may be observed in some lysate(s).

Linkage

Replaces: AB9748

Physical form

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Quality

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