ABC157

Sigma-Aldrich

Anti-MCM7 Antibody

from rabbit

Synonym(s):
DNA replication licensing factor MCM7, CDC47 homolog, P1.1-MCM3
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody product type

primary antibodies

clone

polyclonal

species reactivity

rat, human

species reactivity (predicted by homology)

chimpanzee (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology)

application(s)

western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

General description

MCM7 is a highly conserved mini-chromosome maintenance protein (MCM) that is involved in the initiation of eukaryotic genome replication. It forms a hexameric complex with MCM4 and MCM6 (two subunits of MCM7, two subunits of MCM4 and two subunits of MCM7) and this complex regulates the helicase activity of the pre-replication complex. This complex is also involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. MCM7 is reported to bind to DNA in metaphase II in oocytes and remains associated with the DNA until the S-phase. Two isoforms of MCM7 have been reported, 61 kDa and 90 kDa. In neuroblastoma tumors MCM7 expression is shown to correlates with MYCN (v-myc myelocytomatosis viral related oncogene, neuroblastoma derived) amplification where in MCM7 is rapidly up-regulated by Inducible MYCN Expression. MYCN protein is shown to bind specifically to the MCM7 promoter region, which possesses an E-box binding site for the Myc oncoproteins.

Specificity

Other homologies: Mouse (71% sequence homology).
This antibody recognizes the N-terminus of MCM7

Immunogen

KLH-conjugated linear peptide corresponding to the N-terminus of human MCM7.
Epitope: N-terminus

Application

Research Sub Category
Apoptosis - Additional
Western Blot Analysis: 0.5 µg/mL from a representative lot detected MCM7 in 10 µg of L6 cell lysate.
This MCM7 antibody is validated for use in WB for the detection of the MCM7 protein.
Research Category
Apoptosis & Cancer

Quality

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected MCM7 in 10 µg of HeLa cell lysate.

Target description

~82 kDa observed

Linkage

Replaces: MABE188

Physical form

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein A Purfied

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HeLa cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

MilliporeSigma

Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.