ABD69
Anti-Nestin, Human Antibody
serum, from rabbit
Synonym(s):
Nestin
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 50 μL | Check Cart for Availability | $561.00 |
About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ICC, IHC, WB
Citations:
74
$561.00
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A recombinant, preservative-free antibody is available for your target. Try ZRB69
biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human
should not react with
rat, mouse
technique(s)
immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... NES(10763)
General description
Nestin, a large intermediate filament protein (class Type VI) expressed during development and in myotendinous and neuromuscular junctions. Nestin expression is restricted, typically disappearing by E18. Nestin is thought to be a reasonable neuronal marker; however, recent studies have found nestin expression in other cell types such as endothelial cells {Folia Biologica, 44(5):155, 1998}. Nestin identifies the most primitive neuroepithelium but also identifies many other embryonic tissues, so it is not specific for CNS. Nestin expression is seen in almost all GBMs (Glioblastoma mulitformes) and many melanomas (both primary and metastatic) but not in any metastatic carcinoma.
The anti-Nestin antibody has an observed MW at 220kDa Antibody may see 260kDa band in some cells and/or treatments
Immunogen
GST-tagged recombinant protein corresponding to human Nestin.
Application
Detect Nestin, Human using this Anti-Nestin, Human Antibody validated for use in Western Blotting, ICC & IHC.
Evaluated by Immunocytochemistry in human WI38 cells.
Immunocytochemsitry Analysis: A 1:500 dilution of this antibody detected Nestin in human WI38 cells.
Evaluated by Immunohistochemistry in human glioblastoma tissue.
Immunocytochemsitry Analysis: A 1:2,000 dilution of this antibody detected Nestin in human glioblastoma tissue.
Evaluated by Western Blot in human U251 glioblastoma cells. Western Blot Analysis: A 1:25,000 dilution of this antibody has been shown to detected Nestin in human U251 glioblastoma cells.
Immunocytochemsitry Analysis: A 1:500 dilution of this antibody detected Nestin in human WI38 cells.
Evaluated by Immunohistochemistry in human glioblastoma tissue.
Immunocytochemsitry Analysis: A 1:2,000 dilution of this antibody detected Nestin in human glioblastoma tissue.
Evaluated by Western Blot in human U251 glioblastoma cells. Western Blot Analysis: A 1:25,000 dilution of this antibody has been shown to detected Nestin in human U251 glioblastoma cells.
Biochem/physiol Actions
This antibody recognizes human Nestin. Antibody is not reactive with rat and mouse Nestin protein.
Analysis Note
Control
U251 glioblastoma cells.
U251 glioblastoma cells.
Evaluated by Immunocytochemistry in human U251 glioblastoma cells. Immunocytochemsitry Analysis: A 1:500 dilution of this antibody detected Nestin in human U251 glioblastoma cells.
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
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This Item | |||
|---|---|---|---|
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
| biological source rabbit | biological source mouse | biological source rabbit | biological source mouse |
| antibody form serum | antibody form purified antibody | antibody form affinity isolated antibody | antibody form purified antibody |
| conjugate unconjugated | conjugate CY3 conjugate | conjugate unconjugated | conjugate ALEXA FLUOR™ 488 |
| Gene Information human ... NES(10763) | Gene Information human ... NES(10763) | Gene Information human ... NES(10763) | Gene Information human ... NES(10763) |
| species reactivity human | species reactivity human | species reactivity human (tested) | species reactivity human |
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Storage Class
10 - Combustible liquids
Certificates of Analysis (COA)
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Protocols
Step-by-step culture protocols for neural stem cell culture including NSC isolation, expansion, differentiation and characterization.
Sutapa Ray et al.
Molecular carcinogenesis, 57(4), 536-548 (2017-12-28)
Medulloblastoma (MB) is a malignant pediatric brain tumor with poor prognosis. Signal transducers and activators of transcription-3 (STAT3) is constitutively activated in MB where it functions as an oncoprotein, mediating cancer progression and metastasis. Here, we have delineated the functional
Smita Jagtap et al.
Human molecular genetics, 28(21), 3625-3636 (2019-09-14)
The X-linked neurodevelopmental diseases CDKL5 deficiency disorder (CDD) and Rett syndrome (RTT) are associated with intellectual disability, infantile spasms and seizures. Although mitochondrial dysfunction has been suggested in RTT, less is understood about mitochondrial function in CDD. A comparison of
Intraspinal transplantation of mouse and human neural precursor cells.
Weinger, JG; Chen, L; Coleman, R; Leang, R; Plaisted, WC; Loring, JF; Lane, TE
Current Protocols in Stem Cell Biology null
Global Trade Item Number
| SKU | GTIN |
|---|---|
| ABD69 | 04053252694288 |