MilliporeSigma
All Photos(1)

Documents

ABE2601

Sigma-Aldrich

Anti-DNA helicase B (HELB)

from rabbit

Sign Into View Organizational & Contract Pricing

Select a Size

Change View

Select a Size

Change View
Synonym(s):
DNA helicase B, hDHB
eCl@ss:
32160702

biological source

rabbit

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human

packaging

antibody small pack of 25 μg

technique(s)

western blot: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

target post-translational modification

unmodified

Gene Information

human ... HELB(92797)

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
MABE878MABD34SAB5701545
antibody form

affinity isolated antibody

antibody form

purified antibody

antibody form

purified antibody

antibody form

-

clone

polyclonal

clone

8E3, monoclonal

clone

20A11.1, monoclonal

clone

monoclonal

species reactivity

human

species reactivity

mouse, human

species reactivity

human

species reactivity

human, rat, mouse

packaging

antibody small pack of 25 μg

packaging

-

packaging

-

packaging

-

technique(s)

western blot: suitable

technique(s)

immunocytochemistry: suitable, western blot: suitable

technique(s)

western blot: suitable

technique(s)

immunohistochemistry: 1:50-1:200, western blot: 1:500-1:2000

Still not finding the right product?  

Give our Product Selector Tool a try.

General description

DNA helicase B (UniProt: Q8NG08; also known as EC: 3.6.4.12, hDHB) is encoded by the HELB gene (Gene ID: 92797) in human. DNA helicase B is highly expressed in testis and thymus and weaker expression is observed in liver, spleen, kidney, and brain. It is predominantly nuclear, however, following phosphorylation at serine 967 by CDK2 during the G1/S transition it is exported into the cytoplasm. Following DNA damage, it is recruited to sites of double-strand breaks by the RPA complex. Its recruitment to single-stranded DNA following DNA damage leads to inhibition of nucleases catalyzing resection, such as EXO1, BLM and DNA2, possibly via the 5′-3′ ssDNA translocase activity of HELB. As cells approach S phase, DNA end resection is promoted by the nuclear export of HELB following its phosphorylation. Its nuclear export signal is localized to amino acids 1055-1078. It uses either magnesium or manganese ions to support its helicase activity and the DNA helicase B activity is inhibited by salt concentrations higher than 100 mM. It binds strongly to single-stranded DNA in the absence of ATP, but dissociates readily in the presence of 1 mM ATP. Two isoforms of DNA helicase B have been reported that are produced by alternative splicing.

Specificity

This rabbit polyclonal antibody specifically detects DNA helicase B in human cells.

Immunogen

Epitope: N-terminus
MBP-conjugated recombinant fragment corresponding to the first 199 amino acids from the N-terminal region of human DNA helicase B.

Application

Anti-DNA helicase B (HELB) Antibody, Cat. No. ABE2601, is a highly specific rabbit polyclonal antibody that targets DNA helicase B (HELB) and has been tested for use in Western Blotting.
Research Category
Epigenetics & Nuclear Function
Western Blotting Analysis: A representative lot detected DNA helicase B (HELB) in U2OS cells transfected with a non-targeting siRNA (CTRL) or siRNAs targeting protein and HeLa cells transfected with non-targeting (siCTRL) or HELB-targeting (siHELB) siRNAs (Tkac, J., et. al. (2016). Mol Cell. 61(3):405-418).

Quality

Evaluated by Western Blotting in siRNA control & siRNA HELB U2OS cell lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected DNA helicase B (HELB) in lysate from U2OS cells treated with non-targeting siRNA, but not in lysates from siRNA HELB treated U2OS cells.


Target description

~145 kDa observed; 123.25 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Affinity Purified
Format: Purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service