ABE2604

Sigma-Aldrich

Anti-MCM5

from rabbit

Synonym(s):
DNA replication licensing factor MCM5, CDC46 homolog, P1-CDC46
eCl@ss:
32160702

biological source

rabbit

Quality Level

antibody product type

primary antibodies

clone

polyclonal

species reactivity

Xenopus, human

application(s)

immunohistochemistry: suitable (paraffin)
western blot: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

ambient

General description

DNA replication licensing factor MCM5 (UniProt: P33992; also known as EC:3.6.4.12, CDC46 homolog, P1-CDC46) is encoded by the MCM5 (also known as CDC46) gene (Gene ID: 4174) in human. MCM5 protein as a component of the MCM2-7 (Mini-Chromosome Maintenance; MCM) complex), which consists of six sequence-related AAA + type ATPases/helicases that form a hetero-hexameric ring. It is a putative replicative helicase essential for ′once per cell cycle′ DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. MCM5 is upregulated in the transition from the G0 to G1/S phase of the cell cycle, indicating its active participation in cell cycle regulation. In the G1 phase, inactive MCM2-7 complex is loaded onto origins of DNA replication and during G1-S phase, MCM2-7 complex is activated to unwind the double stranded DNA and plays an important role in DNA replication forks elongation.

Specificity

This polyclonal antibody detects DNA replication licensing factor MCM5 in multiple types of human tissue sections.

Immunogen

A His-tagged full-length recombinant human DNA replication licensing factor MCM5.
Epitope: unknown

Application

Anti-MCM5, Cat. No. ABE2604, is a highly specific rabbit polyclonal antibody that targets DNA replication licensing factor MCM5 and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected MCM5 in human testis and human cervical cancer tissues.

Western Blotting Analysis: A 1:500 dilution of this antibody detected MCM5 in HeLa & CHO cell lysates (Wong, P.G., et. al. (2011). PLoS One. 6(3):e17533; Borysov, S.I., et. al. (2015). Mol Cell Biol. 36(5):832-45).

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected MCM5 in 10 µg of MCF7 cell lysate.
Research Category
Epigenetics & Nuclear Function

Quality

Evaluated by Immunohistochemistry in human tonsil tissue.

Immunohistochemistry Analysis: A 1:250 dilution of this antibody detected MCM5 in human tonsil tissue.

Target description

~90 kDa observed; 82.29 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Unpurified
Unpurified
Rabbit polyclonal antiserum with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin

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