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ABE553

Sigma-Aldrich

Anti-CREB-1 Antibody

from rabbit, purified by affinity chromatography

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Synonym(s):
Cyclic AMP-responsive element-binding protein 1, CREB-1, cAMP-responsive element-binding protein 1
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human, rat

species reactivity (predicted by homology)

porcine (based on 100% sequence homology), equine (based on 100% sequence homology)

technique(s)

ChIP: suitable
immunocytochemistry: suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... CREB1(1385)

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This Item
AB3006AV31264SAB1306301
vibrant-m

ABE553

Anti-CREB-1 Antibody

vibrant-m

AB3006

Anti-CREB Antibody

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

antibody form

IgG fraction of antiserum

antibody form

affinity isolated antibody

biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

rabbit

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

NCBI accession no.

NP_004370

NCBI accession no.

NM_004379.2, NM_134442.2

NCBI accession no.

NP_004370

NCBI accession no.

NP_004370.1

species reactivity

human, rat

species reactivity

human, mouse, rat

species reactivity

mouse, horse, rabbit, rat, dog, human

species reactivity

human

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General description

Cyclic AMP-responsive element-binding protein (CREB) is a beta ZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth and neuronal differentiation in certain neuronal populations. Additionally, CREB signaling is involved in learning and memory in several organisms. CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. CREB is activated by phosphorylation at Ser133 by various signaling pathways including Erk, Ca2+ and stress signaling. Some of the kinases involved in phosphorylating CREB at Ser133 are p90RSK, MSK, CaMKIV, and MAPKAPK-2.

Specificity

This antibody recognizes the N-terminus of CREB-1.

Immunogen

Epitope: N-terminus
KLH-conjugated linear peptide corresponding to the N-terminus of human CREB-1.

Application

Detect CREB using this rabbit polyclonal antibody, Anti-CREB-1 Antibody validated for use in western blotting & ICC.
Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected CREB-1 in PC-12 cells.

Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected CREB-1 in HEK293T cells.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors

Quality

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected CREB-1 in 10 µg of A431 cell lysate.

Target description

~51 kDa observed. A doublet has been observed at 48 kDa in some cell lysates (Rutberg, S. E., et al. (1999). Oncogene. 18(8):1569-1579.). Uncharacterized bands may be observed in some cell lysates.

Linkage

Replaces: 04-218

Physical form

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
A431 cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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