Cell division cycle protein 23 homolog, also known as Anaphase-promoting complex subunit 8 (APC8) or Cyclosome subunit 8, and encoded by the gene name CDC23 or ANAPC8, is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. The individual APC/C components mRNA and protein levels are expressed at approximately the same levels in most tissues and cell lines, suggesting that they perform their functions as part of a complex. In Drosophila, silencing of APC8 results in developmental delay and pupal lethality with elevated levels of apoptosis, high mitotic index, and delayed or blocked mitosis.
KLH-conjugated synthetic peptide corresponding to the center region of human APC8.
Epitope: center region
Immunocytochemistry Analysis: 5 μg/mL from a representative lot detected APC8 in K562 cells.
Research Sub Category
Cell Cycle, DNA Replication & Repair
Detect APC8 using this Anti-APC8 Antibody validated for use in western blotting & ICC.
Epigenetics & Nuclear Function
Evaluated by Western Blotting in K562 cell lysate.
Western Blotting Analysis: 2 μg/mL of this antibody detected APC8 in K562 cell lysate.
~55 kDa observed. Uncharacterized bands may be observed in some lysate(s). Uniprot describes 3 isoforms produced by alternative splicing at ~17 kDa, ~56 kDa, and ~69 kDa
Antigen Affinity Purified
Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
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