ABE797

Sigma-Aldrich

Anti-APC8 Antibody

from rabbit, purified by affinity chromatography

Synonym(s):
Cell division cycle protein 23 homolog, Anaphase-promoting complex subunit 8, APC8, Cyclosome subunit 8
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

application(s)

immunocytochemistry: suitable
western blot: suitable

UniProt accession no.

shipped in

wet ice

General description

Cell division cycle protein 23 homolog, also known as Anaphase-promoting complex subunit 8 (APC8) or Cyclosome subunit 8, and encoded by the gene name CDC23 or ANAPC8, is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. The individual APC/C components mRNA and protein levels are expressed at approximately the same levels in most tissues and cell lines, suggesting that they perform their functions as part of a complex. In Drosophila, silencing of APC8 results in developmental delay and pupal lethality with elevated levels of apoptosis, high mitotic index, and delayed or blocked mitosis.

Immunogen

KLH-conjugated synthetic peptide corresponding to the center region of human APC8.
Epitope: center region

Application

Immunocytochemistry Analysis: 5 μg/mL from a representative lot detected APC8 in K562 cells.
Research Sub Category
Cell Cycle, DNA Replication & Repair
Detect APC8 using this Anti-APC8 Antibody validated for use in western blotting & ICC.
Research Category
Epigenetics & Nuclear Function

Quality

Evaluated by Western Blotting in K562 cell lysate.

Western Blotting Analysis: 2 μg/mL of this antibody detected APC8 in K562 cell lysate.

Target description

~55 kDa observed. Uncharacterized bands may be observed in some lysate(s). Uniprot describes 3 isoforms produced by alternative splicing at ~17 kDa, ~56 kDa, and ~69 kDa

Physical form

Antigen Affinity Purified
Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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