ABE997

Sigma-Aldrich

Anti-MCM3 Antibody Anitbody

serum, from rabbit

Synonym(s):
DNA replication licensing factor MCM3, DNA polymerase alpha holoenzyme-associated protein P1, P1-MCM3, RLF subunit beta, p102
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human, mouse

application(s)

immunocytochemistry: suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

General description

MCM3 (minichromosome maintenance complex component 3) is part of the hexameric MCM DNA helicase, a complex replicative helicase, which plays essential roles in DNA replication.

Immunogen

KLH-conjugated linear peptide corresponding to human MCM3.

Application

Research Sub Category
Transcription Factors
Detect MCM3 using this rabbit polyclonal antibody, Anti-MCM3 Antibody Anitbody validated for use in western blotting & ICC.
Western Blotting Analysis: A representative lot detected MCM3 in IMR90 cell lysate (Chicas, A., et al. (2012). PNAS. 109:(23)8971-8976).
Western Blotting Analysis: A representative lot detected MCM3 in asynchronous culture of Raji cell lysates (Mendes, J., et al. (2000). Molecular and Cellular Biology. 20:(22)8602-8612).
Western Blotting Analysis: A representative lot detected MCM3 in KB cells, derived from human nasopharyngeal epidermoid carcinoma and breast cancer-derived cell lines. (Ekholm-Reed, S., et al. (2004). Journal of Cell Biology. 165(6):789-800).
Immunofluorescence Analysis: A representative lot detected MCM3 in HeLa and MCF-7 cell lysate (Prasanth, et al. (2004). Philosophical Transactions: Biological Sciences. 359:153-154).
Immunofluorescence Analysis: A representative lot detected MCM3 in in KB cells, derived from human nasopharyngeal epidermoid carcinoma and breast cancer-derived cell lines (Ekholm-Reed, S., et al. (2004). Journal of Cell Biology. 165(6):789-800).
Research Category
Epigenetics & Nuclear Function

Quality

Evaluated by Western Blotting in HEK293T cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected MCM3 in 10 µg of HEK293T cell lysate.

Target description

~ 105 kDa observed

Linkage

Replaces: MABE544

Physical form

Unpurified
Rabbit polyclonal in buffer containing serum with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK Germany

WGK 1

J Méndez et al.
Molecular and cellular biology, 20(22), 8602-8612 (2000-10-25)
Evidence obtained from studies with yeast and Xenopus indicate that the initiation of DNA replication is a multistep process. The origin recognition complex (ORC), Cdc6p, and minichromosome maintenance (MCM) proteins are required for establishing prereplication complexes, upon which initiation is...
Susanna Ekholm-Reed et al.
The Journal of cell biology, 165(6), 789-800 (2004-06-16)
Deregulation of cyclin E expression has been associated with a broad spectrum of human malignancies. Analysis of DNA replication in cells constitutively expressing cyclin E at levels similar to those observed in a subset of tumor-derived cell lines indicates that...

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