MilliporeSigma
All Photos(1)

Documents

ABE999

Sigma-Aldrich

Anti-RPB3

from rabbit, purified by affinity chromatography

Sign Into View Organizational & Contract Pricing

Synonym(s):
RPB3, DNA-directed RNA polymerase II subunit RPB3, RNA polymerase II subunit 3, RNA polymerase II subunit B3, DNA-directed RNA polymerase II 33 kDa polypeptide, DNA-directed RNA polymerase II subunit C, RPB31
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human, rat, mouse

species reactivity (predicted by homology)

bovine (based on 100% sequence homology)

technique(s)

western blot: suitable

UniProt accession no.

shipped in

ambient

target post-translational modification

unmodified

Gene Information

human ... POLR2C(5432)

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
ZMS1031ABE30MABE350
Anti-RPB3 from rabbit, purified by affinity chromatography

ABE999

Anti-RPB3

antibody form

affinity isolated antibody

antibody form

purified antibody

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

Quality Level

100

Quality Level

200

Quality Level

100

Quality Level

100

UniProt accession no.

P19387

UniProt accession no.

P24928

UniProt accession no.

P24928

UniProt accession no.

P24928

shipped in

ambient

shipped in

ambient

shipped in

wet ice

shipped in

wet ice

clone

polyclonal

clone

CTD4H8, recombinant monoclonal

clone

polyclonal

clone

3D12, monoclonal

Still not finding the right product?  

Give our Product Selector Tool a try.

General description

RPB3 is one of 12 subunits that make up the complete RNA polymerase, Pol II, the primary RNA polymerase in cellular metabolism. RPB3 is part of the core element of Pol II complex. RPB3 has a central large cleft and the clamp element that moves to open and close the cleft during RNA synthesis. RPB3 naturally localizes to the nucleus and expressed in all cell types and tissues. RPB3 also plays critical roles in RNA splicing and capping, key items of RNA function.

Immunogen

KLH-conjugated linear peptide corresponding to human RPB3 near the Near C-terminus.

Application

Anti-RPB3 Antibody is an antibody against RPB3 for use in Western Blotting.

Quality

Evaluated by Western Blotting in HUVEC cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected RPB3 in 10 µg of HUVEC cell lysate.

Target description

~35 kDa observed

Other Notes

Concentration: Please refer to lot specific datasheet.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Anne M Devlin et al.
Nature communications, 13(1), 7447-7447 (2022-12-03)
Control of RNA Polymerase II (pol II) elongation is a critical component of gene expression in mammalian cells. The PNUTS-PP1 complex controls elongation rates, slowing pol II after polyadenylation sites to promote termination. The Kaposi's sarcoma-associated herpesvirus (KSHV) co-opts pol
Prashant Rawat et al.
Molecular cell, 81(5), 1013-1026 (2021-02-07)
In response to stress, human cells coordinately downregulate transcription and translation of housekeeping genes. To downregulate transcription, the negative elongation factor (NELF) is recruited to gene promoters impairing RNA polymerase II elongation. Here we report that NELF rapidly forms nuclear
Curtis W Bacon et al.
Molecular cell, 78(6), 1133-1151 (2020-05-14)
Precise control of the RNA polymerase II (RNA Pol II) cycle, including pausing and pause release, maintains transcriptional homeostasis and organismal functions. Despite previous work to understand individual transcription steps, we reveal a mechanism that integrates RNA Pol II cycle

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service