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ABF202

Sigma-Aldrich

Anti-IRF7 Antibody

from rabbit, purified by affinity chromatography

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Synonym(s):
Interferon regulatory factor 7, IRF7
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human, mouse

technique(s)

immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... IRF7(3665)

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PRS3941AV38128SAB5700691
vibrant-m

ABF202

Anti-IRF7 Antibody

biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

rabbit

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

UniProt accession no.

Q92985

UniProt accession no.

Q92985

UniProt accession no.

P10914

UniProt accession no.

Q14653

technique(s)

immunofluorescence: suitable, western blot: suitable, immunohistochemistry: suitable

technique(s)

immunofluorescence: suitable, immunohistochemistry: suitable, indirect ELISA: suitable, western blot: suitable

technique(s)

immunohistochemistry: suitable, western blot: suitable

technique(s)

immunohistochemistry: 1:50-1:200, western blot: 1:500-1:2000

Gene Information

human ... IRF7(3665)

Gene Information

human ... IRF7(3665)

Gene Information

human ... IRF1(3659)

Gene Information

human ... IRF3(3661)

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General description

Interferon regulatory factor 7 (IRF7) is a member of the IRF family and has 1 IRF tryptophan pentad repeat DNA-binding domain. IRF7 is key transcriptional regulator of type I interferon (IFN)-dependent immune responses and regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. IRF7 plays an important role in the innate immune response against DNA and RNA viruses and has been shown to activate both the IFN-beta (IFNB) and the IFN-alpha (IFNA) genes and mediate their induction via both the virus-activated, MyD88-independent pathway and the TLR-activated, MyD88-dependent pathway. IRF7 exists in an inactive form in the cytoplasm of uninfected cells and following viral infection it becomes phosphorylated by IKBKE and TBK1 kinases. This leads to its dimerization and nuclear localization where along with other coactivators it can activate transcription of the type I IFN and ISG genes. Studies show that IRF7 induces PSMB9/LMP2 expression, either directly or through induction of IRF1 and may play a role in regulating adaptive immune responses. Other evidence shows that IRF7 binds to the Q promoter (Qp) of EBV nuclear antigen 1 a (EBNA1) and may play a role in the regulation of EBV latency. Additionally, IRF7 is implied to activate distinct gene expression programs in macrophages and regulate the anti-tumor properties of primary macrophages. IRF7 is expressed predominantly in spleen, thymus and peripheral blood leukocytes.

Specificity

This antibody recognizes the C-terminus of IRF7.

Immunogen

Epitope: C-terminus
KLH-conjugated linear peptide corresponding to the C-terminus of human IRF7.

Application

Immunohistochemistry Analysis: 5 µg/mL from a representative lot detected IRF7 in mouse spleen tissue.

Immunofluorescence Analysis: 20 µg/mL from a representative lot detected IRF7 in mouse spleen cells.
Research Category
Inflammation & Immunology
Research Sub Category
Immunological Signaling
This Anti-IRF7 Antibody is validated for use in Western Blotting and Immunohistochemistry and Immunofluorescence for the detection of IRF7.

Quality

Evaluated by Western Blotting in HEK293 whole cell lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected IRF7 in HEK293 whole cell lysate.

Target description

~51 kDa observed. Uncharacterized bands may appear in some lysate(s).

Physical form

Antigen Affinity Purified
Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HEK293 whole cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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