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ABN1480

Sigma-Aldrich

Anti-p47-phox Antibody

from rabbit, purified by affinity chromatography

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Synonym(s):
Neutrophil cytosol factor 1, NCF-1, 47 kDa neutrophil oxidase factor, NCF-47K, Neutrophil NADPH oxidase factor 1, p47-phox, p47-phox
eCl@ss:
32160702

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human, mouse

species reactivity (predicted by homology)

rat (based on 100% sequence homology)

technique(s)

immunohistochemistry: suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

mouse ... Ncf1(17969)

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SAB4504289SAB4504721SAB4502809
antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

species reactivity

human, mouse

species reactivity

rat, mouse, human

species reactivity

rat, human, mouse

species reactivity

human, rat, mouse

technique(s)

immunohistochemistry: suitable, western blot: suitable

technique(s)

ELISA: 1:5000, immunofluorescence: 1:100-1:500, immunohistochemistry: 1:50-1:100, western blot: 1:500-1:1000

technique(s)

ELISA: 1:1000, immunohistochemistry: 1:50-1:100, western blot: 1:500-1:1000

technique(s)

ELISA: 1:1000, immunofluorescence: 1:100-1:500, immunohistochemistry: 1:50-1:100

NCBI accession no.

NP_001272966

NCBI accession no.

P14598

NCBI accession no.

P14598

NCBI accession no.

P14598

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General description

Neutrophil cytosol factor 1 (UniProt Q09014), also known as 47 kDa neutrophil oxidase factor, NADPH oxidase subunit, Ncf-1, NCF-47K, Neutrophil NADPH oxidase factor 1, p47-phox, p47phox) is encoded by the Ncf1 gene in murine species. Activation of NADPH-oxidase dependent superoxide generation requires the participation of several cytosolic factors, including p47-phox, p67-phox, p40-phox and Rac. In the resting cell, p47phox, p67phox, and p40phox form a large molecular weight cytosolic complex. Activation of the NADPH oxidase is initiated by the assembly of p47phox, p67phox, and Rac with cytochrome b558 in a 1:1:1:1 complex in the plasma membrane.

Immunogen

KLH-conjugated linear peptide corresponding to the C-terminus of mouse p47-phox.

Application

Anti-p47-phox Antibody is an antibody against p47-phox for use in Western Blotting, Immunohistochemistry.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected p47-phox in mouse cerebral cortex and mouse small intestine.
Research Category
Neuroscience
Research Sub Category
Developmental Signaling

Quality

Evaluated by Western Blotting in Raji cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected p47-phox in 10 µg of Raji cell lysate.

Target description

~47 kDa observed

Physical form

Affinity purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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J Duncombe et al.
Neuropathology and applied neurobiology, 43(6), 477-491 (2017-01-01)
Normal neurovascular coupling, mediated by the fine interplay and communication of cells within the neurovascular unit, is critical for maintaining normal brain activity and cognitive function. This study investigated whether, with advancing age there is disruption of neurovascular coupling and

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