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ABN475

Sigma-Aldrich

Anti-Wnt Antibody-10b

from rabbit, purified by affinity chromatography

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Synonym(s):
Protein Wnt-10b, Protein Wnt-12
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

technique(s)

immunohistochemistry: suitable
western blot: suitable

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... WNT10B(7480)

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This Item
MABN717SAB2103278HPA055048
vibrant-m

ABN475

Anti-Wnt Antibody-10b

vibrant-m

MABN717

Anti-WNT10B Antibody, clone 5A7

biological source

rabbit

biological source

mouse

biological source

rabbit

biological source

rabbit

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

Gene Information

human ... WNT10B(7480)

Gene Information

human ... WNT10B(7480)

Gene Information

human ... WNT10B(7480)

Gene Information

human ... WNT10B(7480)

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

technique(s)

immunohistochemistry: suitable, western blot: suitable

technique(s)

immunofluorescence: suitable, immunohistochemistry: suitable, western blot: suitable

technique(s)

immunohistochemistry: suitable, western blot: suitable

technique(s)

immunoblotting: 0.04-0.4 μg/mL, immunohistochemistry: 1:500-1:1000

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General description

Protein Wnt-10b (Wnt-10b) is a member of the Wnt family that plays an important role in normal development such as regulation of cell fate, patterning during embryogenesis, and neoplastic transformation. Wnt-10b may promote epithelial cell differentiation and hair shaft growth, demonstrating that Wnt-10b has multiple roles in cell growth and differentiation. Mutations in Wnt-10b have been associated with an autosomal receesive disorder known as split-hand/foot malformation type 6 (SHFM6).

Immunogen

KLH-conjugated linear peptide corresponding to human Wnt-10b.

Application

Detect Wnt using this rabbit polyclonal antibody, Anti-Wnt Antibody-10b validated for use in western blotting & IHC.
Immunohistochemistry Analysis: 2.5 µg/mL from a representative lot detected Wnt-10b in human skeletal muscle tissue.

Quality

Evaluated by Western Blotting in human skeletal muscle tissue lysate.

Western Blotting Analysis: 4 µg/mL of this antibody detected Wnt-10b in 15 µg of human skeletal muscle tissue lysate.

Target description

~45 kDa observed. Uncharacterized bands may appear in some lysates.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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705578-5MG-PW

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1000309185

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