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ABN78

Sigma-Aldrich

Anti-NeuN Antibody (rabbit)

from rabbit, purified by affinity chromatography

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Synonym(s):
RNA binding protein fox-1 homolog 3, Fox-1 homolog C, Fox-3, Hexaribonucleotide-binding protein 3, NeuN, Neuronal nuclei
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse, human, snail, rat

technique(s)

immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

General description

RNA binding protein fox-1 homolog 3 also known as NeuN (Neuronal nuclei) is encoded by the Rbfox3 (also known as D11Bwg0517e, Hrnbp3) gene (Gene ID 52897) in murine species. The RNA-binding Fox (Rbfox) family of splicing factors is comprised of three members, Rbfox1 (Fox-1 or A2BP1), Rbfox2 (Fox-2 or RBM9), and Rbfox3 (Fox-3, HRNBP3 or NeuN). Rbfox proteins regulate splicing of many neuronal transcripts (pre-mRNAs) by binding the sequence (U)GCAUG in introns flanking alternative exons. A (U)GCAUG motif that lies downstream of the alternative exon generally promotes Rbfox-dependent exon inclusion, whereas an upstream motif will usually repress exon inclusion. Originally characterized as a marker of post-mitotic neurons and named neuronal nuclei (NeuN), Rbfox3 plays a role in promoting neuronal differentiation through alternative splicing of Numb pre-mRNA during brain development. Rbfox3-knockout in mice is reported to result in defective hippocampal gene expression, defects in synaptic transmission and plasticity in the dentate gyrus, as well as increased seizure susceptibility and decreased anxiety-related behaviors. Likewise, human RBFOX3 gene mutations have been linked to neurodevelopmental delay, cognitive impairments, autistic features, and epilepsy.

Specificity

Anti NeuN antibody from rabbit is a polyclonal antibody that targets NeuN N-terminal region that ends at the start of the RNA recognition motif (RRM). The target region is present in all human, mouse, and rat spliced isoforms reported by UniProt (A6NFN3, Q8BIF2, D4A2H6, A0A0G2K2J4).

Immunogen

Epitope: N-terminus
GST-tagged recombinant mouse NeuN N-terrminal fragment.

Application

Anti-NeuN (rabbit) Antibody, Cat. No. ABN78, is a highly specific rabbit polyclonal antibody that targets NeuN and has been tested in Immunocytochemistry, Immunofluorescence, Immunohistochemistry, and Western Blotting.
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected NeuN in cultured embryonic E18 rat hippocampus neurons.

Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected NeuN-positive neurons in human (cerebellum and cerebral cortex) and mouse (hippocampus) brain tissue sections.

Immunocytochemistry Analysis: A representative lots immunostained 4% paraformaldehyde-fixed neurons isolated from snail brain (Nikolić, L., et al. (2013). J. Exp. Biol. 216(Pt 18):3531-3541).

Immunofluorescence Analysis: Representative lots detected NeuN-positive neurons in frozen brain sections from 4% paraformaldehyde-perfused mice (Cherry, J.D., et al. (2015). J. Neuroinflammation. 12:203; Huang, B., et al. (2015). Neuron. 85(6):1212-1226; Ataka, K., et al. (2013). PLoS One. 8(11):e81744).

Immunofluorescence Analysis: A representative lots detected NeuN-positive neurons in formalin-fixed and paraffin-embedded amyotrophic lateral sclerosis (ALS) human brain tissue sections (Fratta, P., et al. (2015). Neurobiol. Aging. 36(1):546.e1-7).

Immunohistochemistry Analysis: A representative lot detected NeuN-positive neurons in rat brain tissue sections (Mendonça, M.C., et al. (2013).Toxins. 5(12):2572-2588).

Western Blotting Analysis: Representative lot detected NeuN in mouse and rat brain tissue lysates (Huang, B., et al. (2015). Neuron. 85(6):1212-1226; Mendonça, M.C., et al. (2013).Toxins. 5(12):2572-2588).

This rabbit polyclonal antibody is also avaiable as Alexa Fluor 488 (ABN78A4), biotin (ABN78B), and Cy3 (ABN78C3) conjugates for immunocytochemistry, immunofluorescence, immunohistochemistry applications.
Research Category
Neuroscience
Research Sub Category
Developmental Signaling

Quality

Evaluated by Western Blotting in mouse brain nuclear extract.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected NeuN in 10 µg of mouse brain nuclear extract.

Target description

~48/42 kDa observed. 33.87/35.12 kDa (human isoform 1/2), 40.61/39.05/35.64/34.07/35.63 kDa (mouse isoform 1/2/3/4/5), 40.59kDa (rat; UniProt D4A2H6), 34.05 kDa (rat; UniProt A0A0G2K2J4) calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Affinity purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Mouse brain nuclear extract

Other Notes

Concentration: Please refer to lot specific datasheet.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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